TY - JOUR
T1 - Oral L-glutamine pretreatment attenuates skeletal muscle atrophy induced by 24-h fasting in mice
AU - de Vasconcelos, Diogo Antonio Alves
AU - Giesbertz, Pieter
AU - de Souza, Diego Ribeiro
AU - Vitzel, Kaio Fernando
AU - Abreu, Phablo
AU - Marzuca-Nassr, Gabriel Nasri
AU - Fortes, Marco Aurélio Salomão
AU - Murata, Gilson Masahiro
AU - Hirabara, Sandro Massao
AU - Curi, Rui
AU - Daniel, Hannelore
AU - Pithon-Curi, Tania Cristina
N1 - Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/8
Y1 - 2019/8
N2 - L-Glutamine (L-Gln) supplementation has been pointed out as an anticatabolic intervention, but its effects on protein synthesis and degradation signaling in skeketal muscle are still poorly known. The effects of L-Gln pretreatment (1 g kg−1 day−1 body weight for 10 days) on muscle fiber cross-sectional area (CSA), amino acid composition (measured by LC-MS/MS) and protein synthesis (Akt-mTOR) and degradation (ubiquitin ligases) signaling in soleus and extensor digitorum longus (EDL) muscles in 24-h-fasted mice were investigated. The fiber CSA of EDL muscle was not different between the L-Gln-fasted and L-Gln-fed groups. This finding was associated with reduced contents of L-Leu and L-Iso and activation of protein synthesis signaling (p-RPS6Ser240/244 and Akt-mTOR). The spectrum of soleus muscle fiber CSA distribution was larger in L-Gln-fasted as compared with placebo-fasted mice. This effect of L-Gln pretreatment was associated with changes in red fibers L-Gln metabolism as indicated by increased intracellular L-glutamine/L-glutamate ratio, L-aspartate and GABA levels. L-Gln supplementation reduced fasting-induced mass loss in tibialis anterior and gastrocnemius muscles. Evidence is presented that pretreatment with L-glutamine attenuates skeletal muscle atrophy induced by 24-h fasting through mechanisms that vary with the muscle fiber type.
AB - L-Glutamine (L-Gln) supplementation has been pointed out as an anticatabolic intervention, but its effects on protein synthesis and degradation signaling in skeketal muscle are still poorly known. The effects of L-Gln pretreatment (1 g kg−1 day−1 body weight for 10 days) on muscle fiber cross-sectional area (CSA), amino acid composition (measured by LC-MS/MS) and protein synthesis (Akt-mTOR) and degradation (ubiquitin ligases) signaling in soleus and extensor digitorum longus (EDL) muscles in 24-h-fasted mice were investigated. The fiber CSA of EDL muscle was not different between the L-Gln-fasted and L-Gln-fed groups. This finding was associated with reduced contents of L-Leu and L-Iso and activation of protein synthesis signaling (p-RPS6Ser240/244 and Akt-mTOR). The spectrum of soleus muscle fiber CSA distribution was larger in L-Gln-fasted as compared with placebo-fasted mice. This effect of L-Gln pretreatment was associated with changes in red fibers L-Gln metabolism as indicated by increased intracellular L-glutamine/L-glutamate ratio, L-aspartate and GABA levels. L-Gln supplementation reduced fasting-induced mass loss in tibialis anterior and gastrocnemius muscles. Evidence is presented that pretreatment with L-glutamine attenuates skeletal muscle atrophy induced by 24-h fasting through mechanisms that vary with the muscle fiber type.
KW - 4E-BP1
KW - GABA
KW - L-Glutamate
KW - Muscle fiber type
KW - RPS6
UR - http://www.scopus.com/inward/record.url?scp=85067583148&partnerID=8YFLogxK
U2 - 10.1016/j.jnutbio.2019.05.010
DO - 10.1016/j.jnutbio.2019.05.010
M3 - Article
C2 - 31233980
AN - SCOPUS:85067583148
SN - 0955-2863
VL - 70
SP - 202
EP - 214
JO - Journal of Nutritional Biochemistry
JF - Journal of Nutritional Biochemistry
ER -