Optimization of light and sound delivery for in vivo whole-brain optoacoustic angiography of rodents

Pavel Subochev, Ekaterina Sergeeva, Mikhail Kirillin, Daria Kurakina, Anna Orlova, Alexey Kurnikov, Xosé Luís Deán-Ben, Zhenyue Chen, Daniel Razansky

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Cerebrovascular imaging of rodents is one of the trending applications of optoacoustics aimed at studying brain activity and pathology. Imaging of deep brain structures is often hindered by sub-optimal arrangement of the light delivery and acoustic detection systems. In our work we revisit the physics behind opto-acoustic signal generation and perform theoretical evaluation of optimal laser wavelengths with an aim of enabling whole brain cerebrovascular optoacoustic angiography in small rodents. A comprehensive model based on diffusion approximation was developed to simulate optoacoustic signal generation using optical and acoustic parameters closely mimicking a typical murine brain and surrounding tissues. The model revealed three characteristic wavelength ranges in the visible and near-infrared spectra optimally suited for imaging cerebral vasculature of different size and depth. The theoretical conclusions are confirmed by numerical simulations while in vivo imaging experiments further validated the capacity for accurately resolving brain vasculature at depths ranging between 0.7 and 7 mm.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2021
EditorsAlexander A. Oraevsky, Lihong V. Wang
PublisherSPIE
ISBN (Electronic)9781510641198
DOIs
StatePublished - 2021
Externally publishedYes
EventPhotons Plus Ultrasound: Imaging and Sensing 2021 - Virtual, Online, United States
Duration: 6 Mar 202111 Mar 2021

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11642
ISSN (Print)1605-7422

Conference

ConferencePhotons Plus Ultrasound: Imaging and Sensing 2021
Country/TerritoryUnited States
CityVirtual, Online
Period6/03/2111/03/21

Keywords

  • cerebrovascular imaging
  • matrix array
  • optoacoustic angiography
  • ultrawideband ultrasonic detection

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