Optical mesoscopy without the scatter: Broadband multispectral optoacoustic mesoscopy

Andrei Chekkoury, Jérôme Gateau, Wouter Driessen, Panagiotis Symvoulidis, Nicolas Bézière, Annette Feuchtinger, Axel Walch, Vasilis Ntziachristos

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Optical mesoscopy extends the capabilities of biological visualization beyond the limited penetration depth achieved by microscopy. However, imaging of opaque organisms or tissues larger than a few hundred micrometers requires invasive tissue sectioning or chemical treatment of the specimen for clearing photon scattering, an invasive process that is regardless limited with depth. We developed previously unreported broadband optoacoustic mesoscopy as a tomographic modality to enable imaging of optical contrast through several millimeters of tissue, without the need for chemical treatment of tissues. We show that the unique combination of three-dimensional projections over a broad 500 kHz–40 MHz frequency range combined with multi-wavelength illumination is necessary to render broadband multispectral optoacoustic mesoscopy (2BMSOM) superior to previous optical or optoacoustic mesoscopy implementations.

Original languageEnglish
Article numberA038
Pages (from-to)3134-3148
Number of pages15
JournalBiomedical Optics Express
Volume6
Issue number9
DOIs
StatePublished - 2015

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