TY - JOUR
T1 - On the interaction of adriamycin with DNA
T2 - Investigation of spectral changes
AU - Youngman, Richard J.
AU - Elstner, Erich F.
N1 - Funding Information:
The authors are grateful to the Deutsche For-schungsgemeinschaffot r financial support,a nd to Dr. Charles E. Myers and colleaguesa t the National Cancer Institute, NIH, Bethesda,f or stimulating discussions.
PY - 1984/6
Y1 - 1984/6
N2 - The aerobic reduction of adriamycin by NADPH-cytochrome c-(ferredoxin)oxidoreductase was determined spectrophotometrically and found to consist of an initial slow phase, followed by a rapid stage. Superoxide was found to play a role in the reduction of the quinone drug only during the first phase. The second, faster stage of the reaction was not inhibited by superoxide dismutase, apparently due to the decreased oxygen tension in the reaction cuvette. When adriamycin was fully bound to DNA, no direct reduction by the enzymatic system was observed. However, in the presence of a superoxide-generating system, reduction of the adriamycin-DNA complex did occur.
AB - The aerobic reduction of adriamycin by NADPH-cytochrome c-(ferredoxin)oxidoreductase was determined spectrophotometrically and found to consist of an initial slow phase, followed by a rapid stage. Superoxide was found to play a role in the reduction of the quinone drug only during the first phase. The second, faster stage of the reaction was not inhibited by superoxide dismutase, apparently due to the decreased oxygen tension in the reaction cuvette. When adriamycin was fully bound to DNA, no direct reduction by the enzymatic system was observed. However, in the presence of a superoxide-generating system, reduction of the adriamycin-DNA complex did occur.
UR - http://www.scopus.com/inward/record.url?scp=0021275594&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(84)90406-5
DO - 10.1016/0003-9861(84)90406-5
M3 - Article
C2 - 6428316
AN - SCOPUS:0021275594
SN - 0003-9861
VL - 231
SP - 424
EP - 429
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -