Occurrence of SARS-CoV-2 in the intraocular milieu

Wolfgang List; Peter Regitnig; Karl Kashofer; Gregor Gorkiewicz; Martin Zacharias; Andreas Wedrich; Laura Posch-Pertl

doi:10.1016/j.exer.2020.108273

Occurrence of SARS-CoV-2 in the intraocular milieu

Wolfgang List, Peter Regitnig, Karl Kashofer, Gregor Gorkiewicz, Martin Zacharias, Andreas Wedrich, Laura Posch-Pertl

Medical University of Graz

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The purpose of this research is to study the intraocular occurrence of SARS-CoV-2. In postmortem examinations, aqueous humor and the vitreous samples were collected. All individuals were previously positive in nasopharyngeal swabbing and cause of death was respiratory failure due to SARS-CoV-2 infection. Testing was done using quantitative RT-PCR. We included 16 aqueous humor and 16 vitreous samples for PCR testing. None of the results was positive for SARS-CoV-2. Human GAPDH genes to verify the presence of RNA was present in all aqueous humor samples (16/16, 100%) and 15/16 (93.8%) vitreous samples. In conclusion, this case series found no evidence of SARS-CoV-2 in the intraocular milieu.

Original language	English
Article number	108273
Journal	Experimental Eye Research
Volume	201
DOIs	https://doi.org/10.1016/j.exer.2020.108273
State	Published - Dec 2020
Externally published	Yes

Keywords

Aqueous humor
Intraocular milieu
SARS-CoV-2
Vitreous body

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title = "Occurrence of SARS-CoV-2 in the intraocular milieu",

abstract = "The purpose of this research is to study the intraocular occurrence of SARS-CoV-2. In postmortem examinations, aqueous humor and the vitreous samples were collected. All individuals were previously positive in nasopharyngeal swabbing and cause of death was respiratory failure due to SARS-CoV-2 infection. Testing was done using quantitative RT-PCR. We included 16 aqueous humor and 16 vitreous samples for PCR testing. None of the results was positive for SARS-CoV-2. Human GAPDH genes to verify the presence of RNA was present in all aqueous humor samples (16/16, 100%) and 15/16 (93.8%) vitreous samples. In conclusion, this case series found no evidence of SARS-CoV-2 in the intraocular milieu.",

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T1 - Occurrence of SARS-CoV-2 in the intraocular milieu

AU - List, Wolfgang

AU - Regitnig, Peter

AU - Kashofer, Karl

AU - Gorkiewicz, Gregor

AU - Zacharias, Martin

AU - Wedrich, Andreas

AU - Posch-Pertl, Laura

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AB - The purpose of this research is to study the intraocular occurrence of SARS-CoV-2. In postmortem examinations, aqueous humor and the vitreous samples were collected. All individuals were previously positive in nasopharyngeal swabbing and cause of death was respiratory failure due to SARS-CoV-2 infection. Testing was done using quantitative RT-PCR. We included 16 aqueous humor and 16 vitreous samples for PCR testing. None of the results was positive for SARS-CoV-2. Human GAPDH genes to verify the presence of RNA was present in all aqueous humor samples (16/16, 100%) and 15/16 (93.8%) vitreous samples. In conclusion, this case series found no evidence of SARS-CoV-2 in the intraocular milieu.

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Occurrence of SARS-CoV-2 in the intraocular milieu

Abstract

Keywords

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