Novel Prokaryotic CRISPR-Cas12a-Based Tool for Programmable Transcriptional Activation and Repression

Keyphrases

• Transcriptional Repression 100%
• Transcriptional Activation 100%
• Paenibacillus Polymyxa 100%
• CRISPR-Cas12a 100%
• Nuclease 66%
• Host Organisms 66%
• Guide RNA (gRNA) 66%
• Alternative Host 66%
• Escherichia Coli 33%
• Targeted Sequencing 33%
• Gene Expression 33%
• Transcriptional Regulation 33%
• Gene Knockout 33%
• Multiplexing 33%
• Multi-target 33%
• Polymer Composition 33%
• Metabolic Engineering 33%
• Multiplex 33%
• Prokaryotes 33%
• Lactate Dehydrogenase 33%
• Fermentation Process 33%
• Simultaneous Expression 33%
• Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) 33%
• 2,3-butanediol Dehydrogenase 33%
• Exopolysaccharide Biosynthesis 33%
• Bacterial Cells 33%
• Eukaryotic Organisms 33%
• Anaerobic Fermentation 33%
• Rare Example 33%
• Fluorescent Reporter 33%
• CRISPR Interference (CRISPRi) 33%
• Cas12a 33%
• Reporter Assay 33%
• DCas9 33%
• Transcriptional Perturbation 33%
• Butanediol 33%
• Bacterial Cell Factory 33%
• Lactate Formation 33%
• Genetic Code Reprogramming 33%

Biochemistry, Genetics and Molecular Biology

• Transactivation 100%
• CRISPR-Cas12A 100%
• Transcription Initiation 100%
• Polymyxa 100%
• Nuclease 100%
• Genetics 50%
• Anabolism 50%
• Dehydrogenase 50%
• CRISPR Interference 50%
• Cas9 50%
• Reprogramming 50%
• CRISPR 50%
• Gene Expression 50%
• Escherichia coli 50%
• Fermentation 50%
• Metabolic Engineering 50%
• Paenibacillus polymyxa 50%
• Guide RNA 50%
• Oxidoreductase 50%
• Lactate Dehydrogenase 50%
• Genomics 50%
• Prokaryote 50%

Immunology and Microbiology

• Clustered Regularly Interspaced Short Palindromic Repeat 100%
• Transcription Initiation 100%
• Transactivation 100%
• Nuclease 66%
• Bacterial Cell 66%
• Polymyxa 66%
• Prokaryote 33%
• Gene Expression 33%
• Escherichia coli 33%
• Metabolic Engineering 33%
• Fermentation 33%
• Paenibacillus polymyxa 33%