Novel mutations of mitochondrial complex I in pathologically proven Parkinson disease

Siegfried Kösel, Eva M. Grasbon-Frodl, Ulrike Mautsch, Rupert Egensperger, Ulrich Von Eitzen, Dimitrij Frishman, Sabine Hofmann, Klaus Dieter Gerbitz, Parviz Mehraein, Manuel B. Graeber

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75 Scopus citations


Complete sequence analysis of all mitochondrial complex I genes was performed in 22 cases of neuropathologically confirmed idiopathic Parkinson disease (PD). DNA from the substantia nigra was used as a template for polymerase chain reaction-based genomic sequencing. Seven novel mutations causing the exchange of amino acids were detected in subunit genes ND1 (3992 C/ T, 4024 A/G), ND4 (11253 T/C, 12084 C/T), ND5 (13711 G/A, 13768 T/C), and ND6 (14582 T/C). In addition, five known missense mutations affecting the ND1 (3335 T/C, 3338 T/C), ND2 (5460 G/A), ND3 (10398 A/G), and ND5 (13966 A/G) genes as well as three secondary LHON mutations (4216 T/C, 4917 A/ G, 13708 G/A) were found in the PD group. Among the novel mutations, the 11253 T/C transition which changes a conserved isoleucine residue into threonine is most likely to be of functional relevance. Furthermore, 43 synonymous polymorphisms were detected in PD brains, including 20 novel sequence variants. Haplogroup analysis revealed that most unique missense mutations were found in PD cases belonging to the Dc haplogroup. Our data are in line with the view that PD is not a single disease entity but comprises a genetically heterogeneous group of disorders. The results of our study further suggest that 90% or more of all idiopathic PD cases are not due to sequence variation of mitochondria! complex I, but that mitochondrial mutations may play a pathogenic role in a subset of PD patients.

Original languageEnglish
Pages (from-to)197-204
Number of pages8
Issue number3
StatePublished - 1998
Externally publishedYes


  • Genetic heterogeneity
  • Mitochondrial haplogroups
  • NADH dehydrogenase
  • Susceptibility genes


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