TY - JOUR
T1 - Novel modes of protein-RNA recognition in the RNAi pathway
AU - Lingel, Andreas
AU - Sattler, Michael
PY - 2005/2
Y1 - 2005/2
N2 - Gene silencing mediated by RNA interference (RNAi) depends on short interfering RNAs (siRNAs) and micro RNAs (miRNAs). These RNAs have unique features, namely a defined size of 19-21 base pairs, and characteristic two-nucleotide single-stranded 3′ overhangs and 5′ monophosphate groups. These molecular features of siRNAs and miRNAs are produced by RNase III enzymes, which are a hallmark of gene silencing induced by double-stranded RNA. Recent structural studies of components of the RNAi pathway, including PAZ, Piwi and RNase III domains, as well as full-length Argonaute and viral p19 proteins, have revealed distinct and novel modes of sequence-independent recognition of the characteristic features of siRNAs and miRNAs in the RNAi pathway.
AB - Gene silencing mediated by RNA interference (RNAi) depends on short interfering RNAs (siRNAs) and micro RNAs (miRNAs). These RNAs have unique features, namely a defined size of 19-21 base pairs, and characteristic two-nucleotide single-stranded 3′ overhangs and 5′ monophosphate groups. These molecular features of siRNAs and miRNAs are produced by RNase III enzymes, which are a hallmark of gene silencing induced by double-stranded RNA. Recent structural studies of components of the RNAi pathway, including PAZ, Piwi and RNase III domains, as well as full-length Argonaute and viral p19 proteins, have revealed distinct and novel modes of sequence-independent recognition of the characteristic features of siRNAs and miRNAs in the RNAi pathway.
UR - http://www.scopus.com/inward/record.url?scp=13844254120&partnerID=8YFLogxK
U2 - 10.1016/j.sbi.2005.01.010
DO - 10.1016/j.sbi.2005.01.010
M3 - Review article
C2 - 15718141
AN - SCOPUS:13844254120
SN - 0959-440X
VL - 15
SP - 107
EP - 115
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
IS - 1 SPEC. ISS.
ER -