TY - JOUR
T1 - Novel concepts for the immunological detection of bound residues
AU - Weller, Michael G.
AU - Zeck, Anne
AU - Pfortner, Peter
AU - Simon, Elfriede
AU - Niessner, Reinhard
N1 - Funding Information:
We would like to thank Dr. A.E. Karu, Berkeley, and SDI, Inc., Newark, for the supply of monoclonal antibodies, M. Theisen for TXRF measurements, Dr. R. Loos for HRMS data (both Technical University of Munich), Dr. M. Pischet-srieder for NMR spectra (Ludwig-Maximilians-UniversitMy unich), G. Henkel-mann and Dr. A. Haisch for the triazine contaminated soil samples and the corresponding HPLC data (both Landesanstalt fur Bodenkultur und Pflanzenbau, Munich), the DFG and the Bavarian State Ministry for Nutrition, Agriculture and Forestry for financial support.
PY - 1999
Y1 - 1999
N2 - Immunoassays could be applied successfully to the determination of bound residues in soil and other complex matrices. Nevertheless, there was some doubt whether these assays could be regarded as quantitative and selective. We present some results, which imply that several approaches are quite promising. One approach is based on a non-competitive saturation immunoassay, which evens out the different cross-reactivities of the bound species. This may lead to a true molar sum value, which is not an equivalent concentration. For competitive assays, a method for the determination of the affinity constant(s) of the bound species is discussed. This would enable a correction for the cross-reactivity. The selectivity problem could be diminished very much, too. The application of special blocking reagents and the use of inhibition tests essentially solved the problem of non-specific binding (NSB). In addition, it could be proven experimentally that adsorbed analytes do not disturb the non-competitive assays, which have been suspected to be highly selective for covalently bound residues.
AB - Immunoassays could be applied successfully to the determination of bound residues in soil and other complex matrices. Nevertheless, there was some doubt whether these assays could be regarded as quantitative and selective. We present some results, which imply that several approaches are quite promising. One approach is based on a non-competitive saturation immunoassay, which evens out the different cross-reactivities of the bound species. This may lead to a true molar sum value, which is not an equivalent concentration. For competitive assays, a method for the determination of the affinity constant(s) of the bound species is discussed. This would enable a correction for the cross-reactivity. The selectivity problem could be diminished very much, too. The application of special blocking reagents and the use of inhibition tests essentially solved the problem of non-specific binding (NSB). In addition, it could be proven experimentally that adsorbed analytes do not disturb the non-competitive assays, which have been suspected to be highly selective for covalently bound residues.
KW - Bound residues
KW - Cross-reactivity
KW - Immunoassays
KW - Non-extractable residues
UR - http://www.scopus.com/inward/record.url?scp=0033450902&partnerID=8YFLogxK
U2 - 10.1080/03067319908047311
DO - 10.1080/03067319908047311
M3 - Conference article
AN - SCOPUS:0033450902
SN - 0306-7319
VL - 75
SP - 201
EP - 215
JO - International Journal of Environmental Analytical Chemistry
JF - International Journal of Environmental Analytical Chemistry
IS - 1-2
T2 - The 2nd Euroconference on Environmental Analytical Chemistry
Y2 - 31 October 1998 through 6 November 1998
ER -
