Nitroxide spin labeled RNA for long range distance measurements by EPR-PELDOR

O. Frolow; B. Endeward; O. Schiemann; T. F. Prisner; J. W. Engels

doi:10.1093/nass/nrn078

Nitroxide spin labeled RNA for long range distance measurements by EPR-PELDOR

O. Frolow, B. Endeward, O. Schiemann, T. F. Prisner, J. W. Engels

Johann Wolfgang Goethe University

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Long range distance measurement on RNA allow the determination of RNA folds. Here we report the site specific incorporation of nitroxide spin labels at U,C and A by "on column synthesis". PELDOR (Pulsed Electron Double Resonance) measurements of several RNAs in the range of 2-6 nm were successful.

Original language	English
Pages (from-to)	153-154
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	52
DOIs	https://doi.org/10.1093/nass/nrn078
State	Published - 2008
Externally published	Yes

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10.1093/nass/nrn078

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title = "Nitroxide spin labeled RNA for long range distance measurements by EPR-PELDOR",

abstract = "Long range distance measurement on RNA allow the determination of RNA folds. Here we report the site specific incorporation of nitroxide spin labels at U,C and A by {"}on column synthesis{"}. PELDOR (Pulsed Electron Double Resonance) measurements of several RNAs in the range of 2-6 nm were successful.",

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AU - Frolow, O.

AU - Endeward, B.

AU - Schiemann, O.

AU - Prisner, T. F.

AU - Engels, J. W.

PY - 2008

Y1 - 2008

N2 - Long range distance measurement on RNA allow the determination of RNA folds. Here we report the site specific incorporation of nitroxide spin labels at U,C and A by "on column synthesis". PELDOR (Pulsed Electron Double Resonance) measurements of several RNAs in the range of 2-6 nm were successful.

AB - Long range distance measurement on RNA allow the determination of RNA folds. Here we report the site specific incorporation of nitroxide spin labels at U,C and A by "on column synthesis". PELDOR (Pulsed Electron Double Resonance) measurements of several RNAs in the range of 2-6 nm were successful.

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U2 - 10.1093/nass/nrn078

DO - 10.1093/nass/nrn078

M3 - Article

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AN - SCOPUS:78649425659

SN - 1746-8272

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EP - 154

JO - Nucleic acids symposium series (2004)

JF - Nucleic acids symposium series (2004)

IS - 52

ER -

Nitroxide spin labeled RNA for long range distance measurements by EPR-PELDOR

Abstract

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