Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation

Sabine Radisch, Mateusz P. Poltorak, Michaela Wagner, Vlad Cletiu, Christian Radisch, Irina Treise, Steffi Pann, Alexis Weigt, Sophie Artner, Stefan Dreher, Fabian Fechner, Bojana Borjan, Simon P. Fraessle, Manuel Effenberger, Eileen Benke, Gottfried Navratil, Norbert Hentschel, Dirk H. Busch, Thomas Schmidt, Christian StembergerLothar Germeroth

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Large-scale target cell isolation from patient blood preparations is one of the critical operations during drug product manufacturing for personalized cell therapy in immuno-oncology. Use of high-affinity murine antibody coated magnetic nanoparticles that remain on isolated cells is the current standard applied for this purpose. Here, we present the transformation of previously described technology — non-magnetic immunoaffinity column chromatography-based cell selection with reversible reagents into a new clinical-grade cell isolation platform called Automated Traceless Cell affinity chromatography (ATC). ATC is a fully closed and GMP-compliant cell selection and manufacturing system. Reversibility of reagents enables (sequential) positive cell selection, optionally in combination with depletion columns, enabling capture of highly specific cell subsets. Moreover, synergy with other Streptamer-based technologies allows novel uses beyond cell isolation including integrated and automated on-column target cell activation. In conclusion, ATC technology is an innovative as well as versatile platform to select, stimulate and modify cells for clinical manufacturing and downstream therapies.

Original languageEnglish
Article number6572
JournalScientific Reports
Volume12
Issue number1
DOIs
StatePublished - Dec 2022

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