TY - JOUR
T1 - Neuropotent self-renewing neural stem (NS) cells derived from mouse induced pluripotent stem (iPS) cells
AU - Onorati, Marco
AU - Camnasio, Stefano
AU - Binetti, Maurizio
AU - Jung, Christian B.
AU - Moretti, Alessandra
AU - Cattaneo, Elena
PY - 2010/3
Y1 - 2010/3
N2 - Neural stem (NS) cells are a homogenous population of stem cells that expands in monolayer under serum-free conditions while remaining highly neuropotent. Here, we generated NS cells from induced pluripotent stem (iPS) cells that were previously derived from mouse fibroblasts (NS-(f)iPS). We showed that NS-(f)iPS cells exhibit long-term expansion and express markers of neurogenic radial glia. Analyses of the regional markers expressed in NS-(f)iPS cells suggested a ventral-rhombencephalic identity. Upon exposure to differentiation protocols, NS-(f)iPS cells produce neurons, astrocytes, and oligodendrocytes with an efficiency similar to ES-derived NS cells. NS-(f)iPS cells represent a new tool for studying neural cell fate determination and terminal differentiation, providing an interesting resource for experimental transplantation. Comparative studies between NS cells derived from iPS cells, reprogrammed from different somatic sources, and from authentic ES cells are necessary to identify critical elements for multipotency acquisition.
AB - Neural stem (NS) cells are a homogenous population of stem cells that expands in monolayer under serum-free conditions while remaining highly neuropotent. Here, we generated NS cells from induced pluripotent stem (iPS) cells that were previously derived from mouse fibroblasts (NS-(f)iPS). We showed that NS-(f)iPS cells exhibit long-term expansion and express markers of neurogenic radial glia. Analyses of the regional markers expressed in NS-(f)iPS cells suggested a ventral-rhombencephalic identity. Upon exposure to differentiation protocols, NS-(f)iPS cells produce neurons, astrocytes, and oligodendrocytes with an efficiency similar to ES-derived NS cells. NS-(f)iPS cells represent a new tool for studying neural cell fate determination and terminal differentiation, providing an interesting resource for experimental transplantation. Comparative studies between NS cells derived from iPS cells, reprogrammed from different somatic sources, and from authentic ES cells are necessary to identify critical elements for multipotency acquisition.
KW - Induced pluripotent stem cells
KW - Neural stem cells
KW - Neuronal differentiation
KW - Neuropotency
UR - http://www.scopus.com/inward/record.url?scp=75849152244&partnerID=8YFLogxK
U2 - 10.1016/j.mcn.2009.12.002
DO - 10.1016/j.mcn.2009.12.002
M3 - Article
C2 - 20026276
AN - SCOPUS:75849152244
SN - 1044-7431
VL - 43
SP - 287
EP - 295
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 3
ER -