@article{af69faf9b252491aae9963f1b24cd4d5,
title = "NeuBtracker - Imaging neurobehavioral dynamics in freely behaving fish",
abstract = "A long-standing objective in neuroscience has been to image distributed neuronal activity in freely behaving animals. Here we introduce NeuBtracker, a tracking microscope for simultaneous imaging of neuronal activity and behavior of freely swimming fluorescent reporter fish. We showcase the value of NeuBtracker for screening neurostimulants with respect to their combined neuronal and behavioral effects and for determining spontaneous and stimulus-induced spatiotemporal patterns of neuronal activation during naturalistic behavior.",
author = "Panagiotis Symvoulidis and Antonella Lauri and Anca Stefanoiu and Michele Cappetta and Steffen Schneider and Hongbo Jia and Anja Stelzl and Maximilian Koch and Perez, {Carlos Cruz} and Ahne Myklatun and Sabine Renninger and Andriy Chmyrov and Tobias Lasser and Wolfgang Wurst and Vasilis Ntziachristos and Westmeyer, {Gil G.}",
note = "Funding Information: M. Ahrens (Janelia Research Campus) for sharing tg(HuC:H2B-GCaMP6s), H. Baier and K. Slanchev (Max Planck Institute of Neurobiology) for sharing tg(HuC:Gal4;UAS:eGFP) and tg(HuC:Gal4;UAS:GCaMP6s), and L. Godinho (Technical University of Munich) for providing tg(HuC:Gal4;UAS:GCaMP6s). We are grateful to J. Huisken and F. Fahrbach for providing assistance on building the SPIM; J. Fuchs assisted with the SPIM setup and experiment. We thank B. Wolfrum, P. Rinklin, J. Rebling, D. Razansky and the central machine shop of Helmholtz Zentrum Muenchen for help with the design and production of the arenas; H. Rolbieski for administrative support and help with data organization; T. Durovic and J. Ninkovic for assistance with the two-photon microscopy experiments; M. Fabiszak, C. Penningroth and L. Garrett for valuable comments on the manuscript. We are grateful for support from the European Research Council under grant agreements ERC-StG: 311552 (G.G.W., A.L., P.S.) and the Helmholtz Alliance ICEMED (G.G.W.).",
year = "2017",
month = oct,
day = "31",
doi = "10.1038/nmeth.4459",
language = "English",
volume = "14",
pages = "1079--1082",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Research",
number = "11",
}