@article{a02ccbe99731405b9411720d0f3fd2e0,
title = "Near-infrared branding efficiently correlates light and electron microscopy",
abstract = "The correlation of light and electron microscopy of complex tissues remains a major challenge. Here we report near-infrared branding (NIRB), which facilitates such correlation by using a pulsed, near-infrared laser to create defined fiducial marks in three dimensions in fixed tissue. As these marks are fluorescent and can be photo-oxidized to generate electron contrast, they can guide re-identification of previously imaged structures as small as dendritic spines by electron microscopy.",
author = "Derron Bishop and Ivana Niki{\'c} and Mary Brinkoetter and Sharmon Knecht and Stephanie Potz and Martin Kerschensteiner and Thomas Misgeld",
note = "Funding Information: We thank L. Godinho for critical reading of the manuscript. This work was supported by grants to M.K. and T.M. from the Dana Foundation and the Hertie Foundation and a grant to T.M. and D.B. from the Christopher and Dana Reeve Foundation. D.B. is supported by the US National Institutes of Health. Work in M.K.{\textquoteright}s laboratory is financed through grants from the Deutsche Forschungsgemeinschaft (Emmy-Noether Program, SFB 571 and SFB 870), the German Federal Ministry of Research and Education (Competence Network Multiple Sclerosis) and the {\textquoteleft}Verein Therapieforschung f{\"u}r MS-Kranke e.V.{\textquoteright} T.M. is supported by the Institute of Advanced Study (Technische Universit{\"a}t M{\"u}nchen), the Deutsche Forschungsgemeinschaft (SFB 596), the Bundesministierium f{\"u}r Bildung und Forschung (ERA-Net {\textquoteleft}two-photon imaging{\textquoteright}), the Alexander von Humboldt Foundation and the Center for Integrated Protein Science (Munich).",
year = "2011",
month = jul,
doi = "10.1038/nmeth.1622",
language = "English",
volume = "8",
pages = "568--572",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Research",
number = "7",
}