Nanopore cavity arrays on Silicon-on-Sapphire substrates for optical studies of transport across lipid membranes

Quoc Hung Nguyen, Michael Urban, Robert Tampé, Marc Tornow

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We demonstrate the realization of a novel architecture of nanopore microcavity structures on Silicon-on-Sapphire (SOS) substrates. By advanced nanofabrication and anisotropic wet etching techniques, arrays of femtoliter cavities featuring both, a single nanopore in a top silicon nitride membrane and a transparent bottom, were fabricated. The cavities demonstrated excellent wetting behavior with electrolyte buffer solution and long-term stability of lipid bilayers that were suspended over the nanopores. Preliminary microscopy studies of membrane-enclosed fluorescence markers suggest our new platform for future multiplexed monitoring of membrane transport processes, at single protein resolution.

Original languageEnglish
Title of host publication16th International Conference on Nanotechnology - IEEE NANO 2016
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages74-76
Number of pages3
ISBN (Electronic)9781509039142
DOIs
StatePublished - 21 Nov 2016
Event16th IEEE International Conference on Nanotechnology - IEEE NANO 2016 - Sendai, Japan
Duration: 22 Aug 201625 Aug 2016

Publication series

Name16th International Conference on Nanotechnology - IEEE NANO 2016

Conference

Conference16th IEEE International Conference on Nanotechnology - IEEE NANO 2016
Country/TerritoryJapan
CitySendai
Period22/08/1625/08/16

Keywords

  • Microcavity
  • Nanofabrication
  • Nanopore
  • Silicon-on-sapphire
  • Suspended lipid bilayer

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