Nanometer distance measurements on RNA using PELDOR

Olav Schiemann; Axel Weber; Thomas E. Edwards; Thomas F. Prisner; Snorri T. Sigurdsson

doi:10.1021/ja0274610

Nanometer distance measurements on RNA using PELDOR

Olav Schiemann, Axel Weber, Thomas E. Edwards, Thomas F. Prisner, Snorri T. Sigurdsson

Research output: Contribution to journal › Article › peer-review

111 Scopus citations

Abstract

A rapid increase in RNA structural studies has been seen in the past few years, in part due to the interest in the multitude of cellular functions performed by RNA. Electron paramagnetic resonance spectroscopy is a useful technique for studying biopolymer structure under physiologically relevant conditions. We present herein the use of pulsed electron double resonance for the determination of a 35 ± 2 Å distance between two spin label nitroxides that were linked to specific positions within an RNA.

Original language	English
Pages (from-to)	3434-3435
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	125
Issue number	12
DOIs	https://doi.org/10.1021/ja0274610
State	Published - 26 Mar 2003
Externally published	Yes

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10.1021/ja0274610

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title = "Nanometer distance measurements on RNA using PELDOR",

abstract = "A rapid increase in RNA structural studies has been seen in the past few years, in part due to the interest in the multitude of cellular functions performed by RNA. Electron paramagnetic resonance spectroscopy is a useful technique for studying biopolymer structure under physiologically relevant conditions. We present herein the use of pulsed electron double resonance for the determination of a 35 ± 2 {\AA} distance between two spin label nitroxides that were linked to specific positions within an RNA.",

author = "Olav Schiemann and Axel Weber and Edwards, {Thomas E.} and Prisner, {Thomas F.} and Sigurdsson, {Snorri T.}",

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AU - Schiemann, Olav

AU - Weber, Axel

AU - Edwards, Thomas E.

AU - Prisner, Thomas F.

AU - Sigurdsson, Snorri T.

PY - 2003/3/26

Y1 - 2003/3/26

N2 - A rapid increase in RNA structural studies has been seen in the past few years, in part due to the interest in the multitude of cellular functions performed by RNA. Electron paramagnetic resonance spectroscopy is a useful technique for studying biopolymer structure under physiologically relevant conditions. We present herein the use of pulsed electron double resonance for the determination of a 35 ± 2 Å distance between two spin label nitroxides that were linked to specific positions within an RNA.

AB - A rapid increase in RNA structural studies has been seen in the past few years, in part due to the interest in the multitude of cellular functions performed by RNA. Electron paramagnetic resonance spectroscopy is a useful technique for studying biopolymer structure under physiologically relevant conditions. We present herein the use of pulsed electron double resonance for the determination of a 35 ± 2 Å distance between two spin label nitroxides that were linked to specific positions within an RNA.

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DO - 10.1021/ja0274610

M3 - Article

C2 - 12643697

AN - SCOPUS:0242669315

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

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Nanometer distance measurements on RNA using PELDOR

Abstract

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