Nachweis von Zearalenon-4-β-D-Glucopyranosid in Weizen

Translated title of the contribution: Detection of zearalenone-4-β-D-glucopyranoside in wheat

I. Schneweis, K. Meyer, G. Engelhardt, J. Bauer

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Maize (Zea mays) cell cultures were used for the production of zearalenone-4-β-D-glucopyranoside as standard compound. Wheat samples were extracted with acetonitrile: water, applied to a florisil column and eluted with methanol: ethyl acetate. For determination and quantification of zearalenone-4-β-D-glucopyranoside and zearalenone a LC-MS method was developed. A concentration of 10 μg/kg zearalenone-4-β-D-gluco- pyranoside and zearalenone was detectable. The recovery rates were calculated to be 69% and 89% at a concentration level of 100 μg/kg for zearalenone-4-β-D-glucopyranoside and zearalenone, respectively. 24 Bavarian wheat samples from harvest 1999 were analyzed. Zearalenone was present in 22 out of 24 field samples, the levels ranged from 11-860 μg/kg. Zearalenone-4-β-D-glucopyranoside was found in 10 out of the zearalenone positive samples (42%) at levels ranging from 17 to 104 μg/kg. The amounts of zearalenone-4-β-D-glucopyranoside were correlated to those of zearalenone (r2=0,86; b=0,10).

Translated title of the contributionDetection of zearalenone-4-β-D-glucopyranoside in wheat
Original languageGerman
Pages (from-to)87-91
Number of pages5
JournalMycotoxin Research
Volume17
Issue number1 SUPPL.
DOIs
StatePublished - Mar 2001
Externally publishedYes

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