Abstract
Mucins are high molecular weight membrane glycoproteins. The gene expression of mucins (MUC1-MUC8) may change characteristically during malignant transformation of epithelial tissues. Total RNA was isolated from four bladder cancer cell lines RT4, 647V, HT1376 and 486P (pathological gradings between G1 through G4) and 20 samples of transitional cell carcinomas as well as 16 samples of normal human urothelium of the bladder from surgically removed specimens. The RNA samples were studied with MUC1-, MUC2- and MUC7-specific nested reverse-transcription-polymerase-chain reactions. Gene expression of MUC1 and MUC2 was found positive in all normal as well as in malignant tissue samples and in the tumor cell lines. In contrast, gene expression of MUC7 was only detected in bladder cancer cell lines and samples of invasive transitional cell carcinomas, but neither in superficial, non-invasive bladder tumors nor normal bladder urothelium. Only one of the samples of normal urothelium obtained from 16 different tumor bearing bladders was positive for MUC7 gene expression. These results suggest a differential MUC7 gene expression with the onset of malignant transformations of the bladder urothelium.
| Translated title of the contribution | Evidence of differential mucin 7 (muc7) gene expression in invasive bladder carcinoma |
|---|---|
| Original language | German |
| Pages (from-to) | 46-54 |
| Number of pages | 9 |
| Journal | Journal fur Urologie und Urogynakologie |
| Volume | 6 |
| Issue number | 2 |
| State | Published - 1999 |
| Externally published | Yes |
