Myosin-V stepping kinetics: A molecular model for processivity

Matthias Rief, Ronald S. Rock, Amit D. Mehta, Mark S. Mooseker, Richard E. Cheney, James A. Spudich

Research output: Contribution to journalArticlepeer-review

369 Scopus citations

Abstract

Myosin-V is a molecular motor that moves processively along its actin track. We have used a feedback-enhanced optical trap to examine the stepping kinetics of this movement. By analyzing the distribution of time periods separating discrete ≃36-nm mechanical steps, we characterize the number and duration of rate-limiting biochemical transitions preceding each such step. These data show that myosin-V is a tightly coupled motor whose cycle time is limited by ADP release. On the basis of these results, we propose a model for myosin-V processivity.

Original languageEnglish
Pages (from-to)9482-9486
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number17
DOIs
StatePublished - 15 Aug 2000
Externally publishedYes

Fingerprint

Dive into the research topics of 'Myosin-V stepping kinetics: A molecular model for processivity'. Together they form a unique fingerprint.

Cite this