TY - JOUR
T1 - MYB10 plays a major role in the regulation of flavonoid/phenylpropanoid metabolism during ripening of Fragaria × ananassa fruits
AU - Medina-Puche, Laura
AU - Cumplido-Laso, Guadalupe
AU - Amil-Ruiz, Francisco
AU - Hoffmann, Thomas
AU - Ring, Ludwig
AU - Rodríguez-Franco, Antonio
AU - Caballero, José Luis
AU - Schwab, Wilfried
AU - Muñoz-Blanco, Juan
AU - Blanco-Portales, Rosario
N1 - Funding Information:
This work was supported by a grant from the Ministerio Español de Ciencia e Innovación (EUI2008-03668 and BIO2010-19322). LMP and GCL are grateful for the award of a PhD fellowship within the framework of FPU programme implemented by the Ministerio de Educación y Ciencia and a fellowship from the Comunidad Autonóma de Andalucía, respectively. RBP is grateful for the award of a post-doctoral contract within the framework of the Juan de la Cierva programme implemented by the Ministerio Español de Ciencia e Innovación. LR and WS thank BMBF for financial support in the framework of the FraGenomics project. The authors would also like to thank Nicolás García-Caparrós for his technical assistance in laboratory experiments.
PY - 2014/2
Y1 - 2014/2
N2 - This work characterized the role of the R2R3-MYB10 transcription factor (TF) in strawberry fruit ripening. The expression of this TF takes place mainly in the fruit receptacle and is repressed by auxins and activated by abscisic acid (ABA), in parallel to the ripening process. Anthocyanin was not produced when FaMYB10 expression was transiently silenced in fruit receptacles. An increase in FaMYB10 expression was observed in water-stressed fruits, which was accompanied by an increase in both ABA and anthocyanin content. High-throughput transcriptomic analyses performed in fruits with downregulated FaMYB10 expression indicated that this TF regulates the expression of most of the Early-regulated Biosynthesis Genes (EBGs) and the Late-regulated Biosynthesis Genes (LBGs) genes involved in anthocyanin production in ripened fruit receptacles. Besides, the expression of FaMYB10 was not regulated by FaMYB1 and vice versa. Taken together, all these data clearly indicate that the Fragaria × ananassa MYB10 TF plays a general regulatory role in the flavonoid/phenylpropanoid pathway during the ripening of strawberry
AB - This work characterized the role of the R2R3-MYB10 transcription factor (TF) in strawberry fruit ripening. The expression of this TF takes place mainly in the fruit receptacle and is repressed by auxins and activated by abscisic acid (ABA), in parallel to the ripening process. Anthocyanin was not produced when FaMYB10 expression was transiently silenced in fruit receptacles. An increase in FaMYB10 expression was observed in water-stressed fruits, which was accompanied by an increase in both ABA and anthocyanin content. High-throughput transcriptomic analyses performed in fruits with downregulated FaMYB10 expression indicated that this TF regulates the expression of most of the Early-regulated Biosynthesis Genes (EBGs) and the Late-regulated Biosynthesis Genes (LBGs) genes involved in anthocyanin production in ripened fruit receptacles. Besides, the expression of FaMYB10 was not regulated by FaMYB1 and vice versa. Taken together, all these data clearly indicate that the Fragaria × ananassa MYB10 TF plays a general regulatory role in the flavonoid/phenylpropanoid pathway during the ripening of strawberry
KW - Anthocyanins
KW - flavonoid/phenylpropanoid biosynthesis
KW - fruit receptacle
KW - ripening
KW - strawberry
KW - transcription factor
UR - http://www.scopus.com/inward/record.url?scp=84893244204&partnerID=8YFLogxK
U2 - 10.1093/jxb/ert377
DO - 10.1093/jxb/ert377
M3 - Article
C2 - 24277278
AN - SCOPUS:84893244204
SN - 0022-0957
VL - 65
SP - 401
EP - 417
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
IS - 2
ER -