Murine Models of Salmonella Infection

Gregory T. Walker, Romana R. Gerner, Sean Paul Nuccio, Manuela Raffatellu

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The pathogen Salmonella enterica encompasses a range of bacterial serovars that cause intestinal inflammation and systemic infections in humans. Mice are a widely used infection model due to their relative simplicity and versatility. Here, we provide standardized protocols for culturing the prolific zoonotic pathogen S. enterica serovar Typhimurium for intragastric inoculation of mice to model colitis or systemic dissemination, along with techniques for direct extraintestinal infection. Furthermore, we present procedures for quantifying pathogen burden and for characterizing the immune response by analyzing tissue pathology, inflammatory markers, and immune cells from intestinal tissues.

Original languageEnglish
Article numbere824
JournalCurrent Protocols
Volume3
Issue number7
DOIs
StatePublished - Jul 2023

Keywords

  • Salmonella
  • colitis
  • infection
  • mucosa

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