Abstract
Fluorochromes have become essential reporter molecules in biological research. We show that the depthresolved distribution of fluorochromes in small animals can be imaged with 25fmol sensitivity and 150 μm spatial resolution by means of multispectral photoacoustic imaging. The major advantage of the multispectral approach is the sensitive differentiation of chromophores and fluorochromes of interest based on selfreference measurements, as evidenced in this study by resolving a commonly used fluorochrome (Alexa Fluor 750) in mouse. The suggested method is well suited for enhancing visualization of functional and molecular information in vivo and longitudinally.
| Original language | English |
|---|---|
| Pages (from-to) | 2891-2893 |
| Number of pages | 3 |
| Journal | Optics Letters |
| Volume | 32 |
| Issue number | 19 |
| DOIs | |
| State | Published - 1 Oct 2007 |
| Externally published | Yes |