Multiplexed parallel single transport recordings on nanopore arrays

Alexander Kleefen; Daniel Pedone; Christian Grunwald; Ruoshan Wei; Matthias Firnkes; Gerhard Abstreiter; Ulrich Rant; Robert Tampé

doi:10.1021/nl1033528

Multiplexed parallel single transport recordings on nanopore arrays

Alexander Kleefen, Daniel Pedone, Christian Grunwald, Ruoshan Wei, Matthias Firnkes, Gerhard Abstreiter, Ulrich Rant, Robert Tampé

Natural Sciences

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

We introduce a nanofabricated silicon chip for massively multiplexed analysis of membrane channels and transporters in suspended lipid membranes that does not require any surface modification or organic solvent. Transport processes through single membrane complexes are monitored by fluorescence. The chip consists of an array of well-defined nanopores, addressing an individual pyramidal back-reflecting 30-fL compartment. The setup allows simultaneous analyses of ∼1000 single transmembrane events in one field of view, observing translocation kinetics of transmembrane complexes.

Original language	English
Pages (from-to)	5080-5087
Number of pages	8
Journal	Nano Letters
Volume	10
Issue number	12
DOIs	https://doi.org/10.1021/nl1033528
State	Published - 8 Dec 2010

Keywords

Biosensor
lab-on-chip
membrane proteins
nanofabrication
single channel recording

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10.1021/nl1033528

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AU - Kleefen, Alexander

AU - Pedone, Daniel

AU - Grunwald, Christian

AU - Wei, Ruoshan

AU - Firnkes, Matthias

AU - Abstreiter, Gerhard

AU - Rant, Ulrich

AU - Tampé, Robert

PY - 2010/12/8

Y1 - 2010/12/8

N2 - We introduce a nanofabricated silicon chip for massively multiplexed analysis of membrane channels and transporters in suspended lipid membranes that does not require any surface modification or organic solvent. Transport processes through single membrane complexes are monitored by fluorescence. The chip consists of an array of well-defined nanopores, addressing an individual pyramidal back-reflecting 30-fL compartment. The setup allows simultaneous analyses of ∼1000 single transmembrane events in one field of view, observing translocation kinetics of transmembrane complexes.

AB - We introduce a nanofabricated silicon chip for massively multiplexed analysis of membrane channels and transporters in suspended lipid membranes that does not require any surface modification or organic solvent. Transport processes through single membrane complexes are monitored by fluorescence. The chip consists of an array of well-defined nanopores, addressing an individual pyramidal back-reflecting 30-fL compartment. The setup allows simultaneous analyses of ∼1000 single transmembrane events in one field of view, observing translocation kinetics of transmembrane complexes.

KW - Biosensor

KW - lab-on-chip

KW - membrane proteins

KW - nanofabrication

KW - single channel recording

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JO - Nano Letters

JF - Nano Letters

IS - 12

ER -

Multiplexed parallel single transport recordings on nanopore arrays

Abstract

Keywords

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