TY - JOUR
T1 - Multiplexed electrochemical immunoassay of biomarkers using metal sulfide quantum dot nanolabels and trifunctionalized magnetic beads
AU - Tang, Dianping
AU - Hou, Li
AU - Niessner, Reinhard
AU - Xu, Mingdi
AU - Gao, Zhuangqiang
AU - Knopp, Dietmar
N1 - Funding Information:
This work was financially supported by the National “973” Basic Research Program of China ( 2010CB732403 ), the National Natural Science Foundation of China ( 41176079 , and 21075019 ), the Doctoral Program of Higher Education of China ( 20103514120003 ), the National Science Foundation of Fujian Province ( 2011J06003 ), the China–Russia Bilateral Scientific Cooperation Research Program (NSFC/RFBR) ( 21211120157 ), the Program for Changjiang Scholars and Innovative Research Team in University ( IRT1116 ), and the Alexander von Humboldt-Foundation of Germany .
PY - 2013/8/5
Y1 - 2013/8/5
N2 - A novel multiplexed stripping voltammetric immunoassay protocol was designed for the simultaneous detection of multiple biomarkers (CA 125, CA 15-3, and CA 19-9 used as models) using PAMAM dendrimer-metal sulfide quantum dot (QD) nanolabels as distinguishable signal tags and trifunctionalized magnetic beads as an immunosensing probe. The probe was prepared by means of co-immobilization of primary monoclonal anti-CA 125, anti-CA 15-3 and anti-CA 19-9 antibodies on a single magnetic bead. The PAMAM dendrimer-metal sulfide QD nanolabels containing CdS, ZnS and PbS were synthesized by using in situ synthesis method, which were utilized for the labeling of polyclonal rabbit anti-CA 125, anti-CA 15-3 and anti-CA 19-9 detection antibodies, respectively. A sandwich-type immunoassay format was adopted for the simultaneous determination of target biomarkers in a low-binding microtiter plate. The subsequent anodic stripping voltammetric analysis of cadmium, zinc, and lead components released by acid from the corresponding QD nanolabels was conducted at an in situ prepared mercury film electrode based on the difference of peak potentials. Experimental results indicated that the multiplexed immunoassay enabled the simultaneous detection of three cancer biomarkers in a single run with wide dynamic ranges of 0.01-50UmL-1 and detection limits (LODs) of 0.005UmL-1. Intra-assay and inter-assay coefficients of variation (CVs) were less than 7.2% and 10.4%, respectively. No significant differences at the 0.05 significance level were encountered in the analysis of 10 clinical serum specimens between the multiplexed immunoassay and a commercially available enzyme-linked immunosorbent assay (ELISA).
AB - A novel multiplexed stripping voltammetric immunoassay protocol was designed for the simultaneous detection of multiple biomarkers (CA 125, CA 15-3, and CA 19-9 used as models) using PAMAM dendrimer-metal sulfide quantum dot (QD) nanolabels as distinguishable signal tags and trifunctionalized magnetic beads as an immunosensing probe. The probe was prepared by means of co-immobilization of primary monoclonal anti-CA 125, anti-CA 15-3 and anti-CA 19-9 antibodies on a single magnetic bead. The PAMAM dendrimer-metal sulfide QD nanolabels containing CdS, ZnS and PbS were synthesized by using in situ synthesis method, which were utilized for the labeling of polyclonal rabbit anti-CA 125, anti-CA 15-3 and anti-CA 19-9 detection antibodies, respectively. A sandwich-type immunoassay format was adopted for the simultaneous determination of target biomarkers in a low-binding microtiter plate. The subsequent anodic stripping voltammetric analysis of cadmium, zinc, and lead components released by acid from the corresponding QD nanolabels was conducted at an in situ prepared mercury film electrode based on the difference of peak potentials. Experimental results indicated that the multiplexed immunoassay enabled the simultaneous detection of three cancer biomarkers in a single run with wide dynamic ranges of 0.01-50UmL-1 and detection limits (LODs) of 0.005UmL-1. Intra-assay and inter-assay coefficients of variation (CVs) were less than 7.2% and 10.4%, respectively. No significant differences at the 0.05 significance level were encountered in the analysis of 10 clinical serum specimens between the multiplexed immunoassay and a commercially available enzyme-linked immunosorbent assay (ELISA).
KW - Cancer biomarkers
KW - Electrochemical
KW - Metal sulfide nanolabels
KW - Multiplexed immunoassay
KW - Trifunctionalized magnet beads
UR - http://www.scopus.com/inward/record.url?scp=84875110727&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2013.02.027
DO - 10.1016/j.bios.2013.02.027
M3 - Article
C2 - 23500474
AN - SCOPUS:84875110727
SN - 0956-5663
VL - 46
SP - 37
EP - 43
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -