@article{457ac8f6cd4d43f39736fb0dc6dbee0f,
title = "Multimodal imaging of human cerebellum-merging X-ray phase microtomography, magnetic resonance microscopy and histology",
abstract = "Imaging modalities including magnetic resonance imaging and X-ray computed tomography are established methods in daily clinical diagnosis of human brain. Clinical equipment does not provide sufficient spatial resolution to obtain morphological information on the cellular level, essential for applying minimally or non-invasive surgical interventions. Therefore, generic data with lateral sub-micrometer resolution have been generated from histological slices post mortem. Sub-cellular spatial resolution, lost in the third dimension as a result of sectioning, is obtained using magnetic resonance microscopy and micro computed tomography. We demonstrate that for human cerebellum grating-based X-ray phase tomography shows complementary contrast to magnetic resonance microscopy and histology. In this study, the contrast-to-noise values of magnetic resonance microscopy and phase tomography were comparable whereas the spatial resolution in phase tomography is an order of magnitude better. The registered data with their complementary information permit the distinct segmentation of tissues within the human cerebellum.",
author = "Georg Schulz and Conny Waschkies and Franz Pfeiffer and Irene Zanette and Timm Weitkamp and Christian David and Bert M{\"u}ller",
note = "Funding Information: The authors gratefully acknowledge M. M{\"u}ller-Gerbl and P. Zimmermann for the organization and carrying out the extraction of the human brain, J. Kapfhammer for anatomical and medical information, A. Morel for the brain specimen blocking, M. Imholz for the histology of the specimen, S. Rutishauser for the preparation of the beam-splitter grating, M. Rudin for giving us the opportunity to perform the magnetic resonance microscopy experiments and A. Andronache for making the non-rigid registration tool available. The authors thank M. N. Holme for improving the readability and for correcting the language. T. W. acknowledges support from the French research networks (r{\'e}seau th{\'e}matique de recherche avanc{\'e}e, RTRA) {\textquoteleft}{\textquoteleft}Digiteo{\textquoteright}{\textquoteright} and {\textquoteleft}{\textquoteleft}Triangle de la Physique{\textquoteright}{\textquoteright} (grants 2009-034T and 2009-79D). F. P. acknowledges financial support through the DFG Cluster of Excellence Munich-Center for Advanced Photonics and the European Research Council (FP7, Starting Grant No. 240142). The project was partially funded by the Swiss National Science Foundation (CR23I2_125 406) and was supported by the ESRF (proposal MD-407) through beam time allocation. This work was partly conducted with the support of the Karlsruhe Nano Micro Facility (KNMF), a Helmholtz Research Infrastructure at Karlsruhe Institute of Technology (KIT).",
year = "2012",
doi = "10.1038/srep00826",
language = "English",
volume = "2",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}