Multibranch activity in basal and tuft dendrites during firing of layer 5 cortical neurons in vivo

Daniel N. Hill, Zsuzsanna Varga, Hongbo Ji, Bert Sakmann, Arthur Konnerth

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Layer 5 pyramidal neurons process information from multiple cortical layers to provide a major output of cortex. Because of technical limitations it has remained unclear how these cells integrate widespread synaptic inputs located in distantly separated basal and tuft dendrites. Here, we obtained in vivo twophoton calcium imaging recordings from the entire dendritic field of layer 5 motor cortex neurons. We demonstrate that during subthreshold activity, basal and tuft dendrites exhibit spatially localized, small-amplitude calcium transients reflecting afferent synaptic inputs. During action potential firing, calcium signals in basal dendrites are linearly related to spike activity, whereas calcium signals in the tuft occur unreliably. However, in both dendritic compartments, spike-associated calcium signals were uniformly distributed throughout all branches. Thus, our data support a model of widespread, multibranch integration with a direct impact by basal dendrites and only a partial contribution on output signaling by the tuft.

Original languageEnglish
Pages (from-to)13618-13623
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number33
DOIs
StatePublished - 13 Aug 2013

Keywords

  • Dendritic integration
  • Excitatory synapses
  • Mouse motor cortex

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