TY - JOUR
T1 - Multi-mycotoxin stable isotope dilution LC–MS/MS method for Fusarium toxins in beer
AU - Habler, Katharina
AU - Gotthardt, Marina
AU - Schüler, Jan
AU - Rychlik, Michael
N1 - Publisher Copyright:
© 2016
PY - 2017/3/1
Y1 - 2017/3/1
N2 - A stable isotope dilution LC–MS/MS multi-mycotoxin method was developed for 12 different Fusarium toxins including modified mycotoxins in beer (deoxynivalenol-3-glucoside, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyl-deoxynivalenol, HT2-toxin, T2-toxin, enniatin B, B1, A1, A, beauvericin and zearalenone). As sample preparation and purification of beer a combined solid phase extraction for trichothecenes, enniatins, beauvericin and zearalenone was firstly developed. The validation of the new method gave satisfying results: intra-day and inter-day precision and recoveries were 1–5%, 2–8% and 72–117%, respectively. In total, 61 different organic and conventional beer samples from Germany and all over the world were analyzed by using the newly developed multi-mycotoxin method. In summary, deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyldeoxynivaleneol and enniatin B were quantified in rather low contents in the investigated beer samples. None of the other monitored Fusarium toxins like 15-acetyldeoxynivalenol, HT2- and T2-toxin, zearalenone, enniatin B1, A1, A or beauvericin were detectable.
AB - A stable isotope dilution LC–MS/MS multi-mycotoxin method was developed for 12 different Fusarium toxins including modified mycotoxins in beer (deoxynivalenol-3-glucoside, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyl-deoxynivalenol, HT2-toxin, T2-toxin, enniatin B, B1, A1, A, beauvericin and zearalenone). As sample preparation and purification of beer a combined solid phase extraction for trichothecenes, enniatins, beauvericin and zearalenone was firstly developed. The validation of the new method gave satisfying results: intra-day and inter-day precision and recoveries were 1–5%, 2–8% and 72–117%, respectively. In total, 61 different organic and conventional beer samples from Germany and all over the world were analyzed by using the newly developed multi-mycotoxin method. In summary, deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyldeoxynivaleneol and enniatin B were quantified in rather low contents in the investigated beer samples. None of the other monitored Fusarium toxins like 15-acetyldeoxynivalenol, HT2- and T2-toxin, zearalenone, enniatin B1, A1, A or beauvericin were detectable.
KW - Beer
KW - Fusarium mycotoxins
KW - LC–MS/MS
KW - Solid phase extraction
KW - Stable isotope dilution assay
UR - http://www.scopus.com/inward/record.url?scp=84988407761&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2016.09.100
DO - 10.1016/j.foodchem.2016.09.100
M3 - Article
C2 - 27719934
AN - SCOPUS:84988407761
SN - 0308-8146
VL - 218
SP - 447
EP - 454
JO - Food Chemistry
JF - Food Chemistry
ER -