Multi-mycotoxin stable isotope dilution LC–MS/MS method for Fusarium toxins in beer

Katharina Habler, Marina Gotthardt, Jan Schüler, Michael Rychlik

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

A stable isotope dilution LC–MS/MS multi-mycotoxin method was developed for 12 different Fusarium toxins including modified mycotoxins in beer (deoxynivalenol-3-glucoside, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyl-deoxynivalenol, HT2-toxin, T2-toxin, enniatin B, B1, A1, A, beauvericin and zearalenone). As sample preparation and purification of beer a combined solid phase extraction for trichothecenes, enniatins, beauvericin and zearalenone was firstly developed. The validation of the new method gave satisfying results: intra-day and inter-day precision and recoveries were 1–5%, 2–8% and 72–117%, respectively. In total, 61 different organic and conventional beer samples from Germany and all over the world were analyzed by using the newly developed multi-mycotoxin method. In summary, deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyldeoxynivaleneol and enniatin B were quantified in rather low contents in the investigated beer samples. None of the other monitored Fusarium toxins like 15-acetyldeoxynivalenol, HT2- and T2-toxin, zearalenone, enniatin B1, A1, A or beauvericin were detectable.

Original languageEnglish
Pages (from-to)447-454
Number of pages8
JournalFood Chemistry
Volume218
DOIs
StatePublished - 1 Mar 2017

Keywords

  • Beer
  • Fusarium mycotoxins
  • LC–MS/MS
  • Solid phase extraction
  • Stable isotope dilution assay

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