TY - JOUR
T1 - Morus alba leaf extract
T2 - Metabolite profiling and interactions with antibiotics against Staphylococcus spp. including MRSA
AU - Aelenei, Petruta
AU - Luca, Simon Vlad
AU - Horhogea, Cristina Elena
AU - Rimbu, Cristina Mihaela
AU - Dimitriu, Gabriel
AU - Macovei, Irina
AU - Silion, Mihaela
AU - Aprotosoaie, Ana Clara
AU - Miron, Anca
N1 - Publisher Copyright:
© 2019 Phytochemical Society of Europe
PY - 2019/6
Y1 - 2019/6
N2 - HPLC-DAD-ESI-Q-TOF-MS/MS metabolite profiling of Morus alba leaf extract led to the annotation of 25 constituents (two stilbene derivatives, 11 prenylflavonoids, five 2-arylbenzofurans, seven Diels–Alder type adducts). The extract was found to contain 57.83 mg/g flavonoids, 15.42 mg/g kuwanon G and 10.47 mg/g morusin. Despite its moderate antibacterial activity with minimum inhibitory concentration (MIC) values of 250 μg/mL against methicillin-resistant Staphylococcus aureus (MRSA) ATCC 33591 and ATCC 43300, methicillin-susceptible S. aureus (MSSA) ATCC 6538 and S. epidermidis ATCC 12228, Morus alba leaf extract reversed oxacillin-resistance of MRSA ATCC 43300. In addition, the extract showed synergy with gentamicin against MRSA ATCC 33591 and ATCC 43300 and S. epidermidis ATCC 12228, oxacillin against MRSA ATCC 33591 and tetracycline against S. epidermidis ATCC 12228. In these synergistic combinations, MIC values of antibiotics were significantly decreased (4–32-fold). These findings might be valuable for the development of novel treatment strategies in infections caused by MRSA and S. epidermidis.
AB - HPLC-DAD-ESI-Q-TOF-MS/MS metabolite profiling of Morus alba leaf extract led to the annotation of 25 constituents (two stilbene derivatives, 11 prenylflavonoids, five 2-arylbenzofurans, seven Diels–Alder type adducts). The extract was found to contain 57.83 mg/g flavonoids, 15.42 mg/g kuwanon G and 10.47 mg/g morusin. Despite its moderate antibacterial activity with minimum inhibitory concentration (MIC) values of 250 μg/mL against methicillin-resistant Staphylococcus aureus (MRSA) ATCC 33591 and ATCC 43300, methicillin-susceptible S. aureus (MSSA) ATCC 6538 and S. epidermidis ATCC 12228, Morus alba leaf extract reversed oxacillin-resistance of MRSA ATCC 43300. In addition, the extract showed synergy with gentamicin against MRSA ATCC 33591 and ATCC 43300 and S. epidermidis ATCC 12228, oxacillin against MRSA ATCC 33591 and tetracycline against S. epidermidis ATCC 12228. In these synergistic combinations, MIC values of antibiotics were significantly decreased (4–32-fold). These findings might be valuable for the development of novel treatment strategies in infections caused by MRSA and S. epidermidis.
KW - Antibiotic resistance
KW - Morus alba L.
KW - MRSA
KW - Prenylflavonoids
KW - Synergy
UR - http://www.scopus.com/inward/record.url?scp=85064161850&partnerID=8YFLogxK
U2 - 10.1016/j.phytol.2019.04.006
DO - 10.1016/j.phytol.2019.04.006
M3 - Article
AN - SCOPUS:85064161850
SN - 1874-3900
VL - 31
SP - 217
EP - 224
JO - Phytochemistry Letters
JF - Phytochemistry Letters
ER -