Morphological filtering based on the minkowski functionals in 3D for segmentation of macromolecular structures in intact eukaryotic cells depicted by cryo-electron tomography

H. F. Boehm; F. Jamitzki; R. A. Monetti; D. Mueller; A. Linaroudis; R. Hegerl; E. J. Rummeny; C. W. Raeth

doi:10.1117/12.593717

Morphological filtering based on the minkowski functionals in 3D for segmentation of macromolecular structures in intact eukaryotic cells depicted by cryo-electron tomography

H. F. Boehm, F. Jamitzki, R. A. Monetti, D. Mueller, A. Linaroudis, R. Hegerl, E. J. Rummeny, C. W. Raeth

Former organisations of Medicine and Health

Research output: Contribution to journal › Conference article › peer-review

Abstract

A morphological filtering algorithm based on the Minkowski Functionals in 3D for segmentation of macromolecular structures in intact eukaryotic cells depicted by cryo-electron tomography, was proposed. The 3D image data of an ice-embedded Dictyostelium amoeba directly grown on an electron microscopy grid was analyzed. The specimen is scanned by low dose transmission electron microscopy employing a CM 300 FEG TEM with a single axis tilt series of -50° to + 41.5° at an increment of 1.5°. A 3D reconstruction is obtained by weighted back-projection. Mathematical topology employs additive image functionals, to assign numeric quantities to the shape and connectivity of patterns formed by the pixels in an image.

Original language	English
Article number	62
Pages (from-to)	606-613
Number of pages	8
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	5747
Issue number	I
DOIs	https://doi.org/10.1117/12.593717
State	Published - 2005
Event	Medical Imaging 2005 - Image Processing - San Diego, CA, United States Duration: 13 Feb 2005 → 17 Feb 2005

Keywords

Cryo-electron Tomography
Cytoskeleton
Minkowski Functionals
Segmentation

Access to Document

10.1117/12.593717

Cite this

Boehm, H. F., Jamitzki, F., Monetti, R. A., Mueller, D., Linaroudis, A., Hegerl, R., Rummeny, E. J., & Raeth, C. W. (2005). Morphological filtering based on the minkowski functionals in 3D for segmentation of macromolecular structures in intact eukaryotic cells depicted by cryo-electron tomography. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 5747(I), 606-613. Article 62. https://doi.org/10.1117/12.593717

@article{b1b91347fb1f4d00a7328031c09f25f0,

title = "Morphological filtering based on the minkowski functionals in 3D for segmentation of macromolecular structures in intact eukaryotic cells depicted by cryo-electron tomography",

abstract = "A morphological filtering algorithm based on the Minkowski Functionals in 3D for segmentation of macromolecular structures in intact eukaryotic cells depicted by cryo-electron tomography, was proposed. The 3D image data of an ice-embedded Dictyostelium amoeba directly grown on an electron microscopy grid was analyzed. The specimen is scanned by low dose transmission electron microscopy employing a CM 300 FEG TEM with a single axis tilt series of -50° to + 41.5° at an increment of 1.5°. A 3D reconstruction is obtained by weighted back-projection. Mathematical topology employs additive image functionals, to assign numeric quantities to the shape and connectivity of patterns formed by the pixels in an image.",

keywords = "Cryo-electron Tomography, Cytoskeleton, Minkowski Functionals, Segmentation",

author = "Boehm, {H. F.} and F. Jamitzki and Monetti, {R. A.} and D. Mueller and A. Linaroudis and R. Hegerl and Rummeny, {E. J.} and Raeth, {C. W.}",

year = "2005",

doi = "10.1117/12.593717",

language = "English",

volume = "5747",

pages = "606--613",

journal = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

issn = "1605-7422",

publisher = "SPIE",

number = "I",

note = "Medical Imaging 2005 - Image Processing ; Conference date: 13-02-2005 Through 17-02-2005",

}

Boehm, HF, Jamitzki, F, Monetti, RA, Mueller, D, Linaroudis, A, Hegerl, R, Rummeny, EJ & Raeth, CW 2005, 'Morphological filtering based on the minkowski functionals in 3D for segmentation of macromolecular structures in intact eukaryotic cells depicted by cryo-electron tomography', Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 5747, no. I, 62, pp. 606-613. https://doi.org/10.1117/12.593717

Morphological filtering based on the minkowski functionals in 3D for segmentation of macromolecular structures in intact eukaryotic cells depicted by cryo-electron tomography. / Boehm, H. F.; Jamitzki, F.; Monetti, R. A. et al.
In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 5747, No. I, 62, 2005, p. 606-613.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - Morphological filtering based on the minkowski functionals in 3D for segmentation of macromolecular structures in intact eukaryotic cells depicted by cryo-electron tomography

AU - Boehm, H. F.

AU - Jamitzki, F.

AU - Monetti, R. A.

AU - Mueller, D.

AU - Linaroudis, A.

AU - Hegerl, R.

AU - Rummeny, E. J.

AU - Raeth, C. W.

PY - 2005

Y1 - 2005

N2 - A morphological filtering algorithm based on the Minkowski Functionals in 3D for segmentation of macromolecular structures in intact eukaryotic cells depicted by cryo-electron tomography, was proposed. The 3D image data of an ice-embedded Dictyostelium amoeba directly grown on an electron microscopy grid was analyzed. The specimen is scanned by low dose transmission electron microscopy employing a CM 300 FEG TEM with a single axis tilt series of -50° to + 41.5° at an increment of 1.5°. A 3D reconstruction is obtained by weighted back-projection. Mathematical topology employs additive image functionals, to assign numeric quantities to the shape and connectivity of patterns formed by the pixels in an image.

AB - A morphological filtering algorithm based on the Minkowski Functionals in 3D for segmentation of macromolecular structures in intact eukaryotic cells depicted by cryo-electron tomography, was proposed. The 3D image data of an ice-embedded Dictyostelium amoeba directly grown on an electron microscopy grid was analyzed. The specimen is scanned by low dose transmission electron microscopy employing a CM 300 FEG TEM with a single axis tilt series of -50° to + 41.5° at an increment of 1.5°. A 3D reconstruction is obtained by weighted back-projection. Mathematical topology employs additive image functionals, to assign numeric quantities to the shape and connectivity of patterns formed by the pixels in an image.

KW - Cryo-electron Tomography

KW - Cytoskeleton

KW - Minkowski Functionals

KW - Segmentation

UR - http://www.scopus.com/inward/record.url?scp=23844544432&partnerID=8YFLogxK

U2 - 10.1117/12.593717

DO - 10.1117/12.593717

M3 - Conference article

AN - SCOPUS:23844544432

SN - 1605-7422

VL - 5747

SP - 606

EP - 613

JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

IS - I

M1 - 62

T2 - Medical Imaging 2005 - Image Processing

Y2 - 13 February 2005 through 17 February 2005

ER -

Morphological filtering based on the minkowski functionals in 3D for segmentation of macromolecular structures in intact eukaryotic cells depicted by cryo-electron tomography

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this