Abstract
A monoclonal antibody (K3C6) was developed against Hg(II) and applied in different enzyme immunoassay (EIA) formats to determine the test system with the highest sensitivity. A detection limit of 1.0 μg/L Hg(II) could be achieved with a competitive format in contrast to a detection limit of 2.1 μg/L Hg(II) with a noncompetitive EIA. A competitive displacement EIA yielded the best detection limit of 0.4 μg/L Hg(II) and was well suited to measuring real samples. For this purpose different water samples were diluted at least 1:10 to avoid matrix effects and subsequently spiked with 1 μg/L HgCl2. Recovery of the spiked samples was between 80 and 120%.
| Original language | English |
|---|---|
| Pages (from-to) | 49-52 |
| Number of pages | 4 |
| Journal | Methods |
| Volume | 22 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2000 |