Monitoring the conjugal transfer of plasmid RP4 in activated sludge and in situ identification of the transconjugants

Otto Geisenberger; Aldo Ammendola; Bjarke B. Christensen; Søren Molin; Karl Heinz Schleifer; Leo Eberl

doi:10.1016/S0378-1097(99)00114-7

Monitoring the conjugal transfer of plasmid RP4 in activated sludge and in situ identification of the transconjugants

Otto Geisenberger
, Aldo Ammendola
, Bjarke B. Christensen
, Søren Molin
, Karl Heinz Schleifer
, Leo Eberl

TUM Emeriti of Excellence

Technical University of Munich
Technical University of Denmark

Research output: Contribution to journal › Article › peer-review

75 Scopus citations

Abstract

A GFPmut3b-tagged derivative of broad host-range plasmid RP4 was used to monitor the conjugative transfer of the plasmid from a Pseudomonas putida donor strain to indigenous bacteria in activated sludge. Transfer frequencies were determined to be in the range of 4x10^-6 to 1x10^-5 transconjugants per recipient. In situ hybridisation with fluorescently labeled, rRNA-targeted oligonucleotides was used to phylogenetically affiliate the bacteria that had received the plasmid. Copyright (C) 1999 Federation of European Microbiological Societies.

Original language	English
Pages (from-to)	9-17
Number of pages	9
Journal	FEMS Microbiology Letters
Volume	174
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(99)00114-7
State	Published - 1 May 1999

Keywords

Activated sludge
Green fluorescent protein
Plasmid RP4
Plasmid transfer

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10.1016/S0378-1097(99)00114-7

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title = "Monitoring the conjugal transfer of plasmid RP4 in activated sludge and in situ identification of the transconjugants",

abstract = "A GFPmut3b-tagged derivative of broad host-range plasmid RP4 was used to monitor the conjugative transfer of the plasmid from a Pseudomonas putida donor strain to indigenous bacteria in activated sludge. Transfer frequencies were determined to be in the range of 4x10-6 to 1x10-5 transconjugants per recipient. In situ hybridisation with fluorescently labeled, rRNA-targeted oligonucleotides was used to phylogenetically affiliate the bacteria that had received the plasmid. Copyright (C) 1999 Federation of European Microbiological Societies.",

keywords = "Activated sludge, Green fluorescent protein, Plasmid RP4, Plasmid transfer",

author = "Otto Geisenberger and Aldo Ammendola and Christensen, \{Bjarke B.\} and S{\o}ren Molin and Schleifer, \{Karl Heinz\} and Leo Eberl",

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T1 - Monitoring the conjugal transfer of plasmid RP4 in activated sludge and in situ identification of the transconjugants

AU - Geisenberger, Otto

AU - Ammendola, Aldo

AU - Christensen, Bjarke B.

AU - Molin, Søren

AU - Schleifer, Karl Heinz

AU - Eberl, Leo

PY - 1999/5/1

Y1 - 1999/5/1

N2 - A GFPmut3b-tagged derivative of broad host-range plasmid RP4 was used to monitor the conjugative transfer of the plasmid from a Pseudomonas putida donor strain to indigenous bacteria in activated sludge. Transfer frequencies were determined to be in the range of 4x10-6 to 1x10-5 transconjugants per recipient. In situ hybridisation with fluorescently labeled, rRNA-targeted oligonucleotides was used to phylogenetically affiliate the bacteria that had received the plasmid. Copyright (C) 1999 Federation of European Microbiological Societies.

AB - A GFPmut3b-tagged derivative of broad host-range plasmid RP4 was used to monitor the conjugative transfer of the plasmid from a Pseudomonas putida donor strain to indigenous bacteria in activated sludge. Transfer frequencies were determined to be in the range of 4x10-6 to 1x10-5 transconjugants per recipient. In situ hybridisation with fluorescently labeled, rRNA-targeted oligonucleotides was used to phylogenetically affiliate the bacteria that had received the plasmid. Copyright (C) 1999 Federation of European Microbiological Societies.

KW - Activated sludge

KW - Green fluorescent protein

KW - Plasmid RP4

KW - Plasmid transfer

UR - https://www.scopus.com/pages/publications/0032900709

U2 - 10.1016/S0378-1097(99)00114-7

DO - 10.1016/S0378-1097(99)00114-7

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AN - SCOPUS:0032900709

SN - 0378-1097

VL - 174

SP - 9

EP - 17

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Monitoring the conjugal transfer of plasmid RP4 in activated sludge and in situ identification of the transconjugants

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