Monitoring the conjugal transfer of plasmid RP4 in activated sludge and in situ identification of the transconjugants

Otto Geisenberger; Aldo Ammendola; Bjarke B. Christensen; Søren Molin; Karl Heinz Schleifer; Leo Eberl

doi:10.1016/S0378-1097(99)00114-7

Monitoring the conjugal transfer of plasmid RP4 in activated sludge and in situ identification of the transconjugants

Otto Geisenberger, Aldo Ammendola, Bjarke B. Christensen, Søren Molin, Karl Heinz Schleifer, Leo Eberl

TUM Emeriti of Excellence

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

A GFPmut3b-tagged derivative of broad host-range plasmid RP4 was used to monitor the conjugative transfer of the plasmid from a Pseudomonas putida donor strain to indigenous bacteria in activated sludge. Transfer frequencies were determined to be in the range of 4x10^-6 to 1x10^-5 transconjugants per recipient. In situ hybridisation with fluorescently labeled, rRNA-targeted oligonucleotides was used to phylogenetically affiliate the bacteria that had received the plasmid. Copyright (C) 1999 Federation of European Microbiological Societies.

Original language	English
Pages (from-to)	9-17
Number of pages	9
Journal	FEMS Microbiology Letters
Volume	174
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(99)00114-7
State	Published - 1 May 1999

Keywords

Activated sludge
Green fluorescent protein
Plasmid RP4
Plasmid transfer

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10.1016/S0378-1097(99)00114-7

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title = "Monitoring the conjugal transfer of plasmid RP4 in activated sludge and in situ identification of the transconjugants",

abstract = "A GFPmut3b-tagged derivative of broad host-range plasmid RP4 was used to monitor the conjugative transfer of the plasmid from a Pseudomonas putida donor strain to indigenous bacteria in activated sludge. Transfer frequencies were determined to be in the range of 4x10-6 to 1x10-5 transconjugants per recipient. In situ hybridisation with fluorescently labeled, rRNA-targeted oligonucleotides was used to phylogenetically affiliate the bacteria that had received the plasmid. Copyright (C) 1999 Federation of European Microbiological Societies.",

keywords = "Activated sludge, Green fluorescent protein, Plasmid RP4, Plasmid transfer",

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AU - Geisenberger, Otto

AU - Ammendola, Aldo

AU - Christensen, Bjarke B.

AU - Molin, Søren

AU - Schleifer, Karl Heinz

AU - Eberl, Leo

PY - 1999/5/1

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AB - A GFPmut3b-tagged derivative of broad host-range plasmid RP4 was used to monitor the conjugative transfer of the plasmid from a Pseudomonas putida donor strain to indigenous bacteria in activated sludge. Transfer frequencies were determined to be in the range of 4x10-6 to 1x10-5 transconjugants per recipient. In situ hybridisation with fluorescently labeled, rRNA-targeted oligonucleotides was used to phylogenetically affiliate the bacteria that had received the plasmid. Copyright (C) 1999 Federation of European Microbiological Societies.

KW - Activated sludge

KW - Green fluorescent protein

KW - Plasmid RP4

KW - Plasmid transfer

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JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

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ER -

Monitoring the conjugal transfer of plasmid RP4 in activated sludge and in situ identification of the transconjugants

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