Monitoring the conjugal transfer of plasmid RP4 in activated sludge and in situ identification of the transconjugants

Otto Geisenberger, Aldo Ammendola, Bjarke B. Christensen, Søren Molin, Karl Heinz Schleifer, Leo Eberl

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

A GFPmut3b-tagged derivative of broad host-range plasmid RP4 was used to monitor the conjugative transfer of the plasmid from a Pseudomonas putida donor strain to indigenous bacteria in activated sludge. Transfer frequencies were determined to be in the range of 4x10-6 to 1x10-5 transconjugants per recipient. In situ hybridisation with fluorescently labeled, rRNA-targeted oligonucleotides was used to phylogenetically affiliate the bacteria that had received the plasmid. Copyright (C) 1999 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)9-17
Number of pages9
JournalFEMS Microbiology Letters
Volume174
Issue number1
DOIs
StatePublished - 1 May 1999
Externally publishedYes

Keywords

  • Activated sludge
  • Green fluorescent protein
  • Plasmid RP4
  • Plasmid transfer

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