Monitoring of Toxigenic Cyanobacteria Using Next-Generation Sequencing Techniques

Li Deng; Maxime Sweetlove; Stephan Blank; Dagmar Obbels; Elie Verleyen; Wim Vyverman; Rainer Kurmayer

doi:10.1002/9781119332169.ch10

Monitoring of Toxigenic Cyanobacteria Using Next-Generation Sequencing Techniques

Li Deng, Maxime Sweetlove, Stephan Blank, Dagmar Obbels, Elie Verleyen, Wim Vyverman, Rainer Kurmayer

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

With fast development and wide applications of next generation sequencing (NGS) technologies, pyrosequencing of PCR-amplified fragments that target variable regions within the 16S rRNA gene has quickly become a powerful method for analyzing the microbial community profiles. In this chapter we describe an approach for sequencing 16S rRNA gene amplicons for monitoring of (toxigenic) cyanobacteria and other prokaryotes in environmental samples. Our SOP is particular adapted for relative long amplicon size (~800bp) in order to achieve more accurate taxonomy assignment.

Original language	English
Title of host publication	Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria
Publisher	wiley
Pages	277-299
Number of pages	23
ISBN (Electronic)	9781119332169
ISBN (Print)	9781119332107
DOIs	https://doi.org/10.1002/9781119332169.ch10
State	Published - 1 Jan 2017
Externally published	Yes

Keywords

16S ribosomal RNA gene
amplicon sequencing
community profile
metabarcoding
next-generation sequencing

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10.1002/9781119332169.ch10

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abstract = "With fast development and wide applications of next generation sequencing (NGS) technologies, pyrosequencing of PCR-amplified fragments that target variable regions within the 16S rRNA gene has quickly become a powerful method for analyzing the microbial community profiles. In this chapter we describe an approach for sequencing 16S rRNA gene amplicons for monitoring of (toxigenic) cyanobacteria and other prokaryotes in environmental samples. Our SOP is particular adapted for relative long amplicon size (~800bp) in order to achieve more accurate taxonomy assignment.",

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AU - Deng, Li

AU - Sweetlove, Maxime

AU - Blank, Stephan

AU - Obbels, Dagmar

AU - Verleyen, Elie

AU - Vyverman, Wim

AU - Kurmayer, Rainer

PY - 2017/1/1

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AB - With fast development and wide applications of next generation sequencing (NGS) technologies, pyrosequencing of PCR-amplified fragments that target variable regions within the 16S rRNA gene has quickly become a powerful method for analyzing the microbial community profiles. In this chapter we describe an approach for sequencing 16S rRNA gene amplicons for monitoring of (toxigenic) cyanobacteria and other prokaryotes in environmental samples. Our SOP is particular adapted for relative long amplicon size (~800bp) in order to achieve more accurate taxonomy assignment.

KW - 16S ribosomal RNA gene

KW - amplicon sequencing

KW - community profile

KW - metabarcoding

KW - next-generation sequencing

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M3 - Chapter

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EP - 299

BT - Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria

PB - wiley

ER -

Monitoring of Toxigenic Cyanobacteria Using Next-Generation Sequencing Techniques

Abstract

Keywords

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