Monitoring of cellular behaviour by impedance measurements on interdigitated electrode structures

R. Ehret; W. Baumann; M. Brischwein; A. Schwinde; K. Stegbauer; B. Wolf

doi:10.1016/0956-5663(96)89087-7

Monitoring of cellular behaviour by impedance measurements on interdigitated electrode structures

R. Ehret, W. Baumann, M. Brischwein, A. Schwinde, K. Stegbauer, B. Wolf

University Medical Center

Research output: Contribution to journal › Article › peer-review

365 Scopus citations

Abstract

A new method for on-line and real-time monitoring of concentration, growth and physiological state of cells in culture is described. This biosensor is based on impedance measurements of adherently growing cells on interdigitated electrode structures (IDES). The measurements can be performed for several days as there is no detectable electrical influence on the cells. The versatility of this new sensor is shown with some exemplary experiments. Cell density, growth and long-term behaviour of cells on the electrodes clearly change the impedance of the IDES. Both, the global influence of serum components (deprivation of foetal bovine serum) and the toxic effects of heavy metal ions (cadmium) result in changes of the sensor signal and can be visualized. (C) 1996 Elsevier Science Limited.

Original language	English
Pages (from-to)	29-41
Number of pages	13
Journal	Biosensors and Bioelectronics
Volume	12
Issue number	1
DOIs	https://doi.org/10.1016/0956-5663(96)89087-7
State	Published - 1997
Externally published	Yes

Keywords

Cellular biosensor
Cytotoxicity testing
Impedance
Interdigitated electrode structures
PhysicoControl-Microsystem
Serum components

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10.1016/0956-5663(96)89087-7

Cite this

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abstract = "A new method for on-line and real-time monitoring of concentration, growth and physiological state of cells in culture is described. This biosensor is based on impedance measurements of adherently growing cells on interdigitated electrode structures (IDES). The measurements can be performed for several days as there is no detectable electrical influence on the cells. The versatility of this new sensor is shown with some exemplary experiments. Cell density, growth and long-term behaviour of cells on the electrodes clearly change the impedance of the IDES. Both, the global influence of serum components (deprivation of foetal bovine serum) and the toxic effects of heavy metal ions (cadmium) result in changes of the sensor signal and can be visualized. (C) 1996 Elsevier Science Limited.",

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AU - Ehret, R.

AU - Baumann, W.

AU - Brischwein, M.

AU - Schwinde, A.

AU - Stegbauer, K.

AU - Wolf, B.

PY - 1997

Y1 - 1997

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AB - A new method for on-line and real-time monitoring of concentration, growth and physiological state of cells in culture is described. This biosensor is based on impedance measurements of adherently growing cells on interdigitated electrode structures (IDES). The measurements can be performed for several days as there is no detectable electrical influence on the cells. The versatility of this new sensor is shown with some exemplary experiments. Cell density, growth and long-term behaviour of cells on the electrodes clearly change the impedance of the IDES. Both, the global influence of serum components (deprivation of foetal bovine serum) and the toxic effects of heavy metal ions (cadmium) result in changes of the sensor signal and can be visualized. (C) 1996 Elsevier Science Limited.

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KW - Cytotoxicity testing

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Monitoring of cellular behaviour by impedance measurements on interdigitated electrode structures

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Keywords

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