Monitoring of cellular behaviour by impedance measurements on interdigitated electrode structures

R. Ehret, W. Baumann, M. Brischwein, A. Schwinde, K. Stegbauer, B. Wolf

Research output: Contribution to journalArticlepeer-review

362 Scopus citations

Abstract

A new method for on-line and real-time monitoring of concentration, growth and physiological state of cells in culture is described. This biosensor is based on impedance measurements of adherently growing cells on interdigitated electrode structures (IDES). The measurements can be performed for several days as there is no detectable electrical influence on the cells. The versatility of this new sensor is shown with some exemplary experiments. Cell density, growth and long-term behaviour of cells on the electrodes clearly change the impedance of the IDES. Both, the global influence of serum components (deprivation of foetal bovine serum) and the toxic effects of heavy metal ions (cadmium) result in changes of the sensor signal and can be visualized. (C) 1996 Elsevier Science Limited.

Original languageEnglish
Pages (from-to)29-41
Number of pages13
JournalBiosensors and Bioelectronics
Volume12
Issue number1
DOIs
StatePublished - 1997
Externally publishedYes

Keywords

  • Cellular biosensor
  • Cytotoxicity testing
  • Impedance
  • Interdigitated electrode structures
  • PhysicoControl-Microsystem
  • Serum components

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