Monitoring of assertive Lactobacillus sakei and Lactobacillus curvatus strains using an industrial ring trial experiment

D. Janßen, M. A. Ehrmann, R. F. Vogel

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Aims: In a previous study, we used a 5-day fermenting sausage model to characterize assertiveness of Lactobacillus curvatus and Lactobacillus sakei starter strains towards employ autochthonous contaminants. In this work, we probed those findings and their transferability to real sausage fermentation including the drying process in an industrial ring trial experiment. Methods and Results: Raw fermented sausages (‘salami’) were produced with three L. curvatus and four L. sakei strains as starter cultures in cooperation with three manufacturers from Germany. We monitored pH, water activity and microbiota dynamics at strain level over a total fermentation and ripening time of 21 days by MALDI-TOF-MS identification of isolates. The principal behaviour of the strains in real sausage fermentations was the same as that one observed in the 5-day model system delineating single strain assertiveness of a bacteriocin producer from co-dominance of strains. Conclusions: The water activity decrease, which is concomitant with the sausage ripening process has only limited impact on the assertiveness and survival of the starter strains. Significance and Impact of the Study: Results of a 5-day model can provide insight in the assertiveness of a specific starter strain in sausage fermentation.

Original languageEnglish
Pages (from-to)545-554
Number of pages10
JournalJournal of Applied Microbiology
Volume126
Issue number2
DOIs
StatePublished - Feb 2019

Keywords

  • Lactobacillus curvatus
  • Lactobacillus sakei
  • MALDI-TOF-MS
  • assertiveness
  • lactic acid bacteria
  • raw sausage fermentation

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