Monitoring light scattering characteristics of adherent cell cultures using a lab-on-a-chip

V. Charwat; P. Muellner; R. Hainberger; M. Purtscher; P. Ertl; S. Tedde; O. Hayden

doi:10.1109/ICO-IP.2011.5953790

Monitoring light scattering characteristics of adherent cell cultures using a lab-on-a-chip

V. Charwat, P. Muellner, R. Hainberger, M. Purtscher, P. Ertl, S. Tedde, O. Hayden

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Scopus citations

Abstract

We have developed a -fluidic biochip capable of monitoring in real-time cellular phenotype dynamics by non-invasively assessing cell viability and reproduction over long periods of time. The μ-fluidic biochip contains integrated organic photodiodes that continuously perform light scattering measurements of adherent cell populations. In the presented work, we experimentally show that light scattering measurements are an effective label-free method for accurately assessing cell number variations. Furthermore, results obtained from 3D FDTD simulations indicate that side scatter characteristics of cells allow in principle predictions on cellular health status.

Original language	English
Title of host publication	2011 ICO International Conference on Information Photonics, IP 2011
DOIs	https://doi.org/10.1109/ICO-IP.2011.5953790
State	Published - 2011
Externally published	Yes
Event	2011 ICO International Conference on Information Photonics, IP 2011 - Ottawa, ON, Canada Duration: 18 May 2011 → 20 May 2011

Publication series

Name	2011 ICO International Conference on Information Photonics, IP 2011

Conference

Conference	2011 ICO International Conference on Information Photonics, IP 2011
Country/Territory	Canada
City	Ottawa, ON
Period	18/05/11 → 20/05/11

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10.1109/ICO-IP.2011.5953790

Cite this

Charwat, V., Muellner, P., Hainberger, R., Purtscher, M., Ertl, P., Tedde, S., & Hayden, O. (2011). Monitoring light scattering characteristics of adherent cell cultures using a lab-on-a-chip. In 2011 ICO International Conference on Information Photonics, IP 2011 Article 5953790 (2011 ICO International Conference on Information Photonics, IP 2011). https://doi.org/10.1109/ICO-IP.2011.5953790

@inproceedings{82a48647bdf643198b901e0d187f890e,

title = "Monitoring light scattering characteristics of adherent cell cultures using a lab-on-a-chip",

abstract = "We have developed a -fluidic biochip capable of monitoring in real-time cellular phenotype dynamics by non-invasively assessing cell viability and reproduction over long periods of time. The μ-fluidic biochip contains integrated organic photodiodes that continuously perform light scattering measurements of adherent cell populations. In the presented work, we experimentally show that light scattering measurements are an effective label-free method for accurately assessing cell number variations. Furthermore, results obtained from 3D FDTD simulations indicate that side scatter characteristics of cells allow in principle predictions on cellular health status.",

author = "V. Charwat and P. Muellner and R. Hainberger and M. Purtscher and P. Ertl and S. Tedde and O. Hayden",

year = "2011",

doi = "10.1109/ICO-IP.2011.5953790",

language = "English",

isbn = "9781612843155",

series = "2011 ICO International Conference on Information Photonics, IP 2011",

booktitle = "2011 ICO International Conference on Information Photonics, IP 2011",

note = "2011 ICO International Conference on Information Photonics, IP 2011 ; Conference date: 18-05-2011 Through 20-05-2011",

}

Charwat, V, Muellner, P, Hainberger, R, Purtscher, M, Ertl, P, Tedde, S & Hayden, O 2011, Monitoring light scattering characteristics of adherent cell cultures using a lab-on-a-chip. in 2011 ICO International Conference on Information Photonics, IP 2011., 5953790, 2011 ICO International Conference on Information Photonics, IP 2011, 2011 ICO International Conference on Information Photonics, IP 2011, Ottawa, ON, Canada, 18/05/11. https://doi.org/10.1109/ICO-IP.2011.5953790

Monitoring light scattering characteristics of adherent cell cultures using a lab-on-a-chip. / Charwat, V.; Muellner, P.; Hainberger, R. et al.
2011 ICO International Conference on Information Photonics, IP 2011. 2011. 5953790 (2011 ICO International Conference on Information Photonics, IP 2011).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Monitoring light scattering characteristics of adherent cell cultures using a lab-on-a-chip

AU - Charwat, V.

AU - Muellner, P.

AU - Hainberger, R.

AU - Purtscher, M.

AU - Ertl, P.

AU - Tedde, S.

AU - Hayden, O.

PY - 2011

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N2 - We have developed a -fluidic biochip capable of monitoring in real-time cellular phenotype dynamics by non-invasively assessing cell viability and reproduction over long periods of time. The μ-fluidic biochip contains integrated organic photodiodes that continuously perform light scattering measurements of adherent cell populations. In the presented work, we experimentally show that light scattering measurements are an effective label-free method for accurately assessing cell number variations. Furthermore, results obtained from 3D FDTD simulations indicate that side scatter characteristics of cells allow in principle predictions on cellular health status.

AB - We have developed a -fluidic biochip capable of monitoring in real-time cellular phenotype dynamics by non-invasively assessing cell viability and reproduction over long periods of time. The μ-fluidic biochip contains integrated organic photodiodes that continuously perform light scattering measurements of adherent cell populations. In the presented work, we experimentally show that light scattering measurements are an effective label-free method for accurately assessing cell number variations. Furthermore, results obtained from 3D FDTD simulations indicate that side scatter characteristics of cells allow in principle predictions on cellular health status.

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U2 - 10.1109/ICO-IP.2011.5953790

DO - 10.1109/ICO-IP.2011.5953790

M3 - Conference contribution

AN - SCOPUS:80051768463

SN - 9781612843155

T3 - 2011 ICO International Conference on Information Photonics, IP 2011

BT - 2011 ICO International Conference on Information Photonics, IP 2011

T2 - 2011 ICO International Conference on Information Photonics, IP 2011

Y2 - 18 May 2011 through 20 May 2011

ER -

Monitoring light scattering characteristics of adherent cell cultures using a lab-on-a-chip

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