Molecular markers for cytoplasm in potato: Male sterility and contribution of different plastid-mitochondrial configurations to starch production

A. Lössl, M. Götz, A. Braun, G. Wenzel

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Abstract

Distinct parental cytoplasms were combined in symmetric tetraploid hybrids of potato by somatic cell fusion. This allowed, in the presence of nearly isogenic nuclear genomes, to estimate the contribution of mitochondrial (mt) and chloroplast (cp) genomes to starch content. Analysis of mt-cp configurations in the complete gene pool of german potato cultivars [2n=4x], in a reciprocal dihaploid population [2n=2x], in di-haploid fusion parents [2n=2x] and in their respective hybrids [2n=4x] made visible the effects of different cytoplasmic backgrounds and mitochondrial subgenomic rearrangements. Genotypes identified by markers as cytoplasm Wγ were associated with cytoplasmic male sterility. Evaluation of cytoplasmic types leads to the conclusion, that in starch content the 'wild type' cytoplasms Wα and Wγ, have a significant advantage to other cytoplasmic types (Tβ, Wδ, Sε). This results from the experiments with a reciprocal population, 180 di-haploids, and from cultivar comparisons. In hybrids an interaction between starch content and different mt-cp combinations could be found. In general the highest field performance, measured in starch content and yield was associated with such cytoplasmic configurations which appeared to a high frequency within a population, when the segregation process was completed. This fact is explained by a selection advantage of clones with optimized organellar segregation already during in vitro phase. PCR markers for cytoplasm differentiation are actualized on a website, http://www.flg.tum.de/pbpz/mm/mt/hybrid.html.

Original languageEnglish
Pages (from-to)221-230
Number of pages10
JournalEuphytica
Volume116
Issue number3
DOIs
StatePublished - 2000

Keywords

  • Cytoplasm
  • Male sterility
  • Plastid
  • Potato
  • Somatic hybrids
  • Starch

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