TY - JOUR
T1 - Molecular cytogenetic monitoring from CD34+ peripheral blood cells in myelodysplastic syndromes
T2 - First results from a prospective multicenter German diagnostic study
AU - Braulke, Friederike
AU - Jung, Klaus
AU - Schanz, Julie
AU - Götze, Katharina
AU - Müller-Thomas, Catharina
AU - Platzbecker, Uwe
AU - Germing, Ulrich
AU - Brümmendorf, Tim H.
AU - Bug, Gesine
AU - Ottmann, Oliver
AU - Giagounidis, Aristoteles A.N.
AU - Stadler, Michael
AU - Hofmann, Wolf Karsten
AU - Schafhausen, Philippe
AU - Lübbert, Michael
AU - Schlenk, Richard F.
AU - Blau, Igor W.
AU - Ganster, Christina
AU - Pfeiffer, Sebastian
AU - Shirneshan, Katayoon
AU - Metz, Michael
AU - Detken, Sven
AU - Seraphin, Jörg
AU - Jentsch-Ullrich, Kathleen
AU - Böhme, Angelika
AU - Schmidt, Burkhard
AU - Trümper, Lorenz
AU - Haase, Detlef
N1 - Funding Information:
This study was supported by Celgene ® Germany .
PY - 2013/8
Y1 - 2013/8
N2 - The gold standard of cytogenetic analysis in myelodysplastic syndromes (MDS) is conventional chromosome banding (CCB) analysis of bone marrow (BM) metaphases. Most aberrations can also be detected by fluorescence-in situ-hybridization (FISH). For this prospective multicenter German diagnostic study (www.clinicaltrials.gov: #. NCT01355913) 360 patients, as yet, were followed up to 3 years by sequential FISH analyses of immunomagnetically enriched CD34+ peripheral blood (PB) cells using comprehensive FISH probe panels, resulting in a total number of 19,516 FISH analyses. We demonstrate that CD34+ PB FISH correlates significantly with CCB analysis and represents a feasible method for a reliable non-invasive cytogenetic monitoring from PB.
AB - The gold standard of cytogenetic analysis in myelodysplastic syndromes (MDS) is conventional chromosome banding (CCB) analysis of bone marrow (BM) metaphases. Most aberrations can also be detected by fluorescence-in situ-hybridization (FISH). For this prospective multicenter German diagnostic study (www.clinicaltrials.gov: #. NCT01355913) 360 patients, as yet, were followed up to 3 years by sequential FISH analyses of immunomagnetically enriched CD34+ peripheral blood (PB) cells using comprehensive FISH probe panels, resulting in a total number of 19,516 FISH analyses. We demonstrate that CD34+ PB FISH correlates significantly with CCB analysis and represents a feasible method for a reliable non-invasive cytogenetic monitoring from PB.
KW - CD34+
KW - Chromosomal aberrations
KW - Cytogenetics
KW - FISH
KW - Karyotype evolution
KW - MDS
UR - http://www.scopus.com/inward/record.url?scp=84879788390&partnerID=8YFLogxK
U2 - 10.1016/j.leukres.2013.03.019
DO - 10.1016/j.leukres.2013.03.019
M3 - Article
C2 - 23623559
AN - SCOPUS:84879788390
SN - 0145-2126
VL - 37
SP - 900
EP - 906
JO - Leukemia Research
JF - Leukemia Research
IS - 8
ER -
