Molecular cloning, function prediction and phylogenetic analysis of LMW glutenin subunit genes in Triticum timopheevii (Zhuk.)

M. Y. Zhang, K. Wang, S. L. Wang, X. H. Li, F. J. Zeller, S. L.K. Hsam, Y. M. Yan

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

With 4 figures and 3 tables Two new low-molecular-weight glutenin subunits (LMW-GS) genes from Triticum timopheevii (Zhuk.) were cloned by reverse-transcription PCR (RT-PCR). The complete open reading frames of 897 and 894 bp, corresponding to the size of LMW-GS genes were designated as TTLMW-m1 and TTLMW-m2, respectively. Multiple sequence alignment analysis indicated that these two genes showed high similarity with known LMW-m type genes, albeit possessing distinct sequence variations. A total of 10 single-nucleotide polymorphisms in the TTLMW-m1 and TTLMW-m2 genes were identified, including five non-synonymous mutations. The predicted secondary structure of the deduced mature glutenin subunits of TTLMW-m1 and TTLMW-m2 genes showed that both subunits possessed more α-helixes and β-strands than the control subunit AY263369, suggesting a more viscoelastic dough structure and a positive effect on quality. The homology tree and phylogenetic analysis showed that the two LMW-m type genes were most likely located at the Glu-G3 locus and that they diverged from the LMW-i type genes about 13 million years ago (MYA). The G genome in T. timopheevii appears to be closely related to the D and B genomes of Triticum aestivum.

Original languageEnglish
Pages (from-to)622-629
Number of pages8
JournalPlant Breeding
Volume129
Issue number6
DOIs
StatePublished - Dec 2010

Keywords

  • CDNA
  • LMW-GS genes
  • Secondary structure

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