Molecular characterization of woodchuck interleukin-10 receptor and enhanced function of specific T cells from chronically infected woodchucks following its blockade

Min Jiang; Jia Liu; Ejuan Zhang; Zhongji Meng; Baoju Wang; Michael Roggendorf; Dongliang Yang; Mengji Lu; Yang Xu

doi:10.1016/j.cimid.2012.06.003

Molecular characterization of woodchuck interleukin-10 receptor and enhanced function of specific T cells from chronically infected woodchucks following its blockade

Min Jiang, Jia Liu, Ejuan Zhang, Zhongji Meng, Baoju Wang, Michael Roggendorf, Dongliang Yang, Mengji Lu, Yang Xu

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Interleukin 10 (IL-10) is a pleiotropic cytokine acting on a variety of immune cells through the cell surface receptor (IL-10R). It has been suggested to resuscitate antiviral immunity by interfering with IL-10/IL-10R pathway. The woodchuck model infected by woodchuck hepatitis virus (WHV) represents an informative animal model to study hepatitis B virus (HBV) infection. In this study, the woodchuck IL-10R (wIL-10R) was molecularly cloned and characterized, showing high similarity of its nucleotide and amino acid sequences to that of other mammalian species. The expression level of wIL-10R mRNA in woodchuck peripheral blood mononuclear cells was significantly increased in acute WHV infection but down-regulated during chronic WHV infection. Specific rabbit antibodies against wIL-10R were prepared and showed the ability to enhance the proliferation and degranulation of specific T-cells from chronically WHV-infected woodchucks in vitro. The present work on wIL-10R provided a good basis for future preclinical studies on therapeutic approaches for chronic HBV infection.

Original language	English
Pages (from-to)	563-573
Number of pages	11
Journal	Comparative Immunology, Microbiology and Infectious Diseases
Volume	35
Issue number	6
DOIs	https://doi.org/10.1016/j.cimid.2012.06.003
State	Published - Dec 2012
Externally published	Yes

Keywords

Interleukin 10 receptor
Woodchuck

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.cimid.2012.06.003

Cite this

Jiang, M., Liu, J., Zhang, E., Meng, Z., Wang, B., Roggendorf, M., Yang, D., Lu, M., & Xu, Y. (2012). Molecular characterization of woodchuck interleukin-10 receptor and enhanced function of specific T cells from chronically infected woodchucks following its blockade. Comparative Immunology, Microbiology and Infectious Diseases, 35(6), 563-573. https://doi.org/10.1016/j.cimid.2012.06.003

@article{92093074df6940d3ad57ad1760413061,

title = "Molecular characterization of woodchuck interleukin-10 receptor and enhanced function of specific T cells from chronically infected woodchucks following its blockade",

abstract = "Interleukin 10 (IL-10) is a pleiotropic cytokine acting on a variety of immune cells through the cell surface receptor (IL-10R). It has been suggested to resuscitate antiviral immunity by interfering with IL-10/IL-10R pathway. The woodchuck model infected by woodchuck hepatitis virus (WHV) represents an informative animal model to study hepatitis B virus (HBV) infection. In this study, the woodchuck IL-10R (wIL-10R) was molecularly cloned and characterized, showing high similarity of its nucleotide and amino acid sequences to that of other mammalian species. The expression level of wIL-10R mRNA in woodchuck peripheral blood mononuclear cells was significantly increased in acute WHV infection but down-regulated during chronic WHV infection. Specific rabbit antibodies against wIL-10R were prepared and showed the ability to enhance the proliferation and degranulation of specific T-cells from chronically WHV-infected woodchucks in vitro. The present work on wIL-10R provided a good basis for future preclinical studies on therapeutic approaches for chronic HBV infection.",

keywords = "Interleukin 10 receptor, Woodchuck",

author = "Min Jiang and Jia Liu and Ejuan Zhang and Zhongji Meng and Baoju Wang and Michael Roggendorf and Dongliang Yang and Mengji Lu and Yang Xu",

note = "Funding Information: We thank Thekla Kemper for excellent technical assistance. This work was sponsored by the Science and Technology Project of Wuhan ( 201052399653 ), the Fundamental Research Funds for the Central Universities (HUST: 2012QN140 ) to Y. Xu and grants of Deutsche Forschungsgemeinschaft to M. Lu ( GRK1045/2 and Transregio TRR60).",

year = "2012",

month = dec,

doi = "10.1016/j.cimid.2012.06.003",

language = "English",

volume = "35",

pages = "563--573",

journal = "Comparative Immunology, Microbiology and Infectious Diseases",

issn = "0147-9571",

publisher = "Elsevier Ltd.",

number = "6",

}

Jiang, M, Liu, J, Zhang, E, Meng, Z, Wang, B, Roggendorf, M, Yang, D, Lu, M & Xu, Y 2012, 'Molecular characterization of woodchuck interleukin-10 receptor and enhanced function of specific T cells from chronically infected woodchucks following its blockade', Comparative Immunology, Microbiology and Infectious Diseases, vol. 35, no. 6, pp. 563-573. https://doi.org/10.1016/j.cimid.2012.06.003

Molecular characterization of woodchuck interleukin-10 receptor and enhanced function of specific T cells from chronically infected woodchucks following its blockade. / Jiang, Min; Liu, Jia; Zhang, Ejuan et al.
In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 35, No. 6, 12.2012, p. 563-573.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Molecular characterization of woodchuck interleukin-10 receptor and enhanced function of specific T cells from chronically infected woodchucks following its blockade

AU - Jiang, Min

AU - Liu, Jia

AU - Zhang, Ejuan

AU - Meng, Zhongji

AU - Wang, Baoju

AU - Roggendorf, Michael

AU - Yang, Dongliang

AU - Lu, Mengji

AU - Xu, Yang

N1 - Funding Information: We thank Thekla Kemper for excellent technical assistance. This work was sponsored by the Science and Technology Project of Wuhan ( 201052399653 ), the Fundamental Research Funds for the Central Universities (HUST: 2012QN140 ) to Y. Xu and grants of Deutsche Forschungsgemeinschaft to M. Lu ( GRK1045/2 and Transregio TRR60).

PY - 2012/12

Y1 - 2012/12

N2 - Interleukin 10 (IL-10) is a pleiotropic cytokine acting on a variety of immune cells through the cell surface receptor (IL-10R). It has been suggested to resuscitate antiviral immunity by interfering with IL-10/IL-10R pathway. The woodchuck model infected by woodchuck hepatitis virus (WHV) represents an informative animal model to study hepatitis B virus (HBV) infection. In this study, the woodchuck IL-10R (wIL-10R) was molecularly cloned and characterized, showing high similarity of its nucleotide and amino acid sequences to that of other mammalian species. The expression level of wIL-10R mRNA in woodchuck peripheral blood mononuclear cells was significantly increased in acute WHV infection but down-regulated during chronic WHV infection. Specific rabbit antibodies against wIL-10R were prepared and showed the ability to enhance the proliferation and degranulation of specific T-cells from chronically WHV-infected woodchucks in vitro. The present work on wIL-10R provided a good basis for future preclinical studies on therapeutic approaches for chronic HBV infection.

AB - Interleukin 10 (IL-10) is a pleiotropic cytokine acting on a variety of immune cells through the cell surface receptor (IL-10R). It has been suggested to resuscitate antiviral immunity by interfering with IL-10/IL-10R pathway. The woodchuck model infected by woodchuck hepatitis virus (WHV) represents an informative animal model to study hepatitis B virus (HBV) infection. In this study, the woodchuck IL-10R (wIL-10R) was molecularly cloned and characterized, showing high similarity of its nucleotide and amino acid sequences to that of other mammalian species. The expression level of wIL-10R mRNA in woodchuck peripheral blood mononuclear cells was significantly increased in acute WHV infection but down-regulated during chronic WHV infection. Specific rabbit antibodies against wIL-10R were prepared and showed the ability to enhance the proliferation and degranulation of specific T-cells from chronically WHV-infected woodchucks in vitro. The present work on wIL-10R provided a good basis for future preclinical studies on therapeutic approaches for chronic HBV infection.

KW - Interleukin 10 receptor

KW - Woodchuck

UR - http://www.scopus.com/inward/record.url?scp=84868465676&partnerID=8YFLogxK

U2 - 10.1016/j.cimid.2012.06.003

DO - 10.1016/j.cimid.2012.06.003

M3 - Article

C2 - 22784930

AN - SCOPUS:84868465676

SN - 0147-9571

VL - 35

SP - 563

EP - 573

JO - Comparative Immunology, Microbiology and Infectious Diseases

JF - Comparative Immunology, Microbiology and Infectious Diseases

IS - 6

ER -

Molecular characterization of woodchuck interleukin-10 receptor and enhanced function of specific T cells from chronically infected woodchucks following its blockade

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this