Molecular characterization of the type I IFN receptor in two woodchuck species and detection of its expression in liver samples from woodchucks infected with woodchuck hepatitis virus (WHV)

Hebin Fan; Zhenni Zhu; Yang Wang; Xiaoyong Zhang; Yinping Lu; Yuanqing Tao; Wei Fan; Zhongdong Wang; Hu Wang; Micheal Roggendorf; Mengji Lu; Baoju Wang; Dongliang Yang

doi:10.1016/j.cyto.2012.05.013

Molecular characterization of the type I IFN receptor in two woodchuck species and detection of its expression in liver samples from woodchucks infected with woodchuck hepatitis virus (WHV)

Hebin Fan, Zhenni Zhu, Yang Wang, Xiaoyong Zhang, Yinping Lu, Yuanqing Tao, Wei Fan, Zhongdong Wang, Hu Wang, Micheal Roggendorf, Mengji Lu, Baoju Wang, Dongliang Yang

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Type I interferons (IFN-α/β) serve as the first line of defense against viral infection and share the same type I IFN receptor (IFNAR) complex, which is composed of IFNAR1 and -2. The Eastern woodchuck (Marmota monax) and Chinese woodchuck (Marmota himalayana) are suitable for studying hepatitis B virus (HBV) infection. Here, the complete or partial sequences of the IFNARs of both species were obtained and analyzed. Small interference RNAs targeting wIFNAR1 and -2 specifically down-regulated the expression of wIFNAR1 and -2 and the IFN-stimulated gene MxA in a woodchuck cell line, respectively. IFNAR2 was significantly up-regulated in primary woodchuck hepatocytes stimulated with IFN-α or -γ. The expression of woodchuck IFNAR1 and -2 was decreased in woodchucks chronically infected with woodchuck hepatitis virus (WHV). These results are essential for studying type I IFN-related innate immunity and therapy in hepadnaviral infection in the woodchuck model.

Original language	English
Pages (from-to)	179-185
Number of pages	7
Journal	Cytokine
Volume	60
Issue number	1
DOIs	https://doi.org/10.1016/j.cyto.2012.05.013
State	Published - Oct 2012
Externally published	Yes

Keywords

Marmota himalayana
Marmota monax
Type i interferon receptor
Woodchuck
Woodchuck hepatitis virus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.cyto.2012.05.013

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Fan, H., Zhu, Z., Wang, Y., Zhang, X., Lu, Y., Tao, Y., Fan, W., Wang, Z., Wang, H., Roggendorf, M., Lu, M., Wang, B., & Yang, D. (2012). Molecular characterization of the type I IFN receptor in two woodchuck species and detection of its expression in liver samples from woodchucks infected with woodchuck hepatitis virus (WHV). Cytokine, 60(1), 179-185. https://doi.org/10.1016/j.cyto.2012.05.013

@article{4a08a29ba44941e8b538829ced8512d5,

title = "Molecular characterization of the type I IFN receptor in two woodchuck species and detection of its expression in liver samples from woodchucks infected with woodchuck hepatitis virus (WHV)",

abstract = "Type I interferons (IFN-α/β) serve as the first line of defense against viral infection and share the same type I IFN receptor (IFNAR) complex, which is composed of IFNAR1 and -2. The Eastern woodchuck (Marmota monax) and Chinese woodchuck (Marmota himalayana) are suitable for studying hepatitis B virus (HBV) infection. Here, the complete or partial sequences of the IFNARs of both species were obtained and analyzed. Small interference RNAs targeting wIFNAR1 and -2 specifically down-regulated the expression of wIFNAR1 and -2 and the IFN-stimulated gene MxA in a woodchuck cell line, respectively. IFNAR2 was significantly up-regulated in primary woodchuck hepatocytes stimulated with IFN-α or -γ. The expression of woodchuck IFNAR1 and -2 was decreased in woodchucks chronically infected with woodchuck hepatitis virus (WHV). These results are essential for studying type I IFN-related innate immunity and therapy in hepadnaviral infection in the woodchuck model.",

keywords = "Marmota himalayana, Marmota monax, Type i interferon receptor, Woodchuck, Woodchuck hepatitis virus",

author = "Hebin Fan and Zhenni Zhu and Yang Wang and Xiaoyong Zhang and Yinping Lu and Yuanqing Tao and Wei Fan and Zhongdong Wang and Hu Wang and Micheal Roggendorf and Mengji Lu and Baoju Wang and Dongliang Yang",

note = "Funding Information: This work was supported by grants from the National Major Science and Technology Project for Infectious Diseases of China ( 2008ZX10002-011 ), the State Major Basic Research Program of China ( 2005CB522901, 2007CB512804, 2009CB522506 ), the National Natural Science Foundation of China ( 30271170, 81101248 ), the International Science & Technology Cooperation Program of China ( 2011DFA31030 ), and the Deutsche Forschungsgemeinschaft (Transregio TRR60). ",

year = "2012",

month = oct,

doi = "10.1016/j.cyto.2012.05.013",

language = "English",

volume = "60",

pages = "179--185",

journal = "Cytokine",

issn = "1043-4666",

publisher = "Academic Press",

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}

Fan, H, Zhu, Z, Wang, Y, Zhang, X, Lu, Y, Tao, Y, Fan, W, Wang, Z, Wang, H, Roggendorf, M, Lu, M, Wang, B & Yang, D 2012, 'Molecular characterization of the type I IFN receptor in two woodchuck species and detection of its expression in liver samples from woodchucks infected with woodchuck hepatitis virus (WHV)', Cytokine, vol. 60, no. 1, pp. 179-185. https://doi.org/10.1016/j.cyto.2012.05.013

TY - JOUR

T1 - Molecular characterization of the type I IFN receptor in two woodchuck species and detection of its expression in liver samples from woodchucks infected with woodchuck hepatitis virus (WHV)

AU - Fan, Hebin

AU - Zhu, Zhenni

AU - Wang, Yang

AU - Zhang, Xiaoyong

AU - Lu, Yinping

AU - Tao, Yuanqing

AU - Fan, Wei

AU - Wang, Zhongdong

AU - Wang, Hu

AU - Roggendorf, Micheal

AU - Lu, Mengji

AU - Wang, Baoju

AU - Yang, Dongliang

N1 - Funding Information: This work was supported by grants from the National Major Science and Technology Project for Infectious Diseases of China ( 2008ZX10002-011 ), the State Major Basic Research Program of China ( 2005CB522901, 2007CB512804, 2009CB522506 ), the National Natural Science Foundation of China ( 30271170, 81101248 ), the International Science & Technology Cooperation Program of China ( 2011DFA31030 ), and the Deutsche Forschungsgemeinschaft (Transregio TRR60).

PY - 2012/10

Y1 - 2012/10

N2 - Type I interferons (IFN-α/β) serve as the first line of defense against viral infection and share the same type I IFN receptor (IFNAR) complex, which is composed of IFNAR1 and -2. The Eastern woodchuck (Marmota monax) and Chinese woodchuck (Marmota himalayana) are suitable for studying hepatitis B virus (HBV) infection. Here, the complete or partial sequences of the IFNARs of both species were obtained and analyzed. Small interference RNAs targeting wIFNAR1 and -2 specifically down-regulated the expression of wIFNAR1 and -2 and the IFN-stimulated gene MxA in a woodchuck cell line, respectively. IFNAR2 was significantly up-regulated in primary woodchuck hepatocytes stimulated with IFN-α or -γ. The expression of woodchuck IFNAR1 and -2 was decreased in woodchucks chronically infected with woodchuck hepatitis virus (WHV). These results are essential for studying type I IFN-related innate immunity and therapy in hepadnaviral infection in the woodchuck model.

AB - Type I interferons (IFN-α/β) serve as the first line of defense against viral infection and share the same type I IFN receptor (IFNAR) complex, which is composed of IFNAR1 and -2. The Eastern woodchuck (Marmota monax) and Chinese woodchuck (Marmota himalayana) are suitable for studying hepatitis B virus (HBV) infection. Here, the complete or partial sequences of the IFNARs of both species were obtained and analyzed. Small interference RNAs targeting wIFNAR1 and -2 specifically down-regulated the expression of wIFNAR1 and -2 and the IFN-stimulated gene MxA in a woodchuck cell line, respectively. IFNAR2 was significantly up-regulated in primary woodchuck hepatocytes stimulated with IFN-α or -γ. The expression of woodchuck IFNAR1 and -2 was decreased in woodchucks chronically infected with woodchuck hepatitis virus (WHV). These results are essential for studying type I IFN-related innate immunity and therapy in hepadnaviral infection in the woodchuck model.

KW - Marmota himalayana

KW - Marmota monax

KW - Type i interferon receptor

KW - Woodchuck

KW - Woodchuck hepatitis virus

UR - http://www.scopus.com/inward/record.url?scp=84865413083&partnerID=8YFLogxK

U2 - 10.1016/j.cyto.2012.05.013

DO - 10.1016/j.cyto.2012.05.013

M3 - Article

C2 - 22705153

AN - SCOPUS:84865413083

SN - 1043-4666

VL - 60

SP - 179

EP - 185

JO - Cytokine

JF - Cytokine

IS - 1

ER -

Molecular characterization of the type I IFN receptor in two woodchuck species and detection of its expression in liver samples from woodchucks infected with woodchuck hepatitis virus (WHV)

Abstract

Keywords

UN SDGs

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