Molecular characterization of the Sinorhizobium meliloti nlpD gene

Wolfgang R. Streit, Kai Hofmann, Wolfgang Liebl

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The Sinorhizobium meliloti nlpD gene consists of 1,539 nucleotides and codes for 512 amino acids. Expression of the nlpD gene as a histidine-tagged protein in Escherichia coli resulted in the production of a 57-kDa protein. The deduced polypeptide sequence of NlpD contains one unusual hexamer repeat (KVQRGQ), one tetramer (TVTV) and two direct and inverted trimer repeats (KAA, AAK). The N-terminal amino acid residues displayed similarity with signal peptides of secreted bacterial lipoproteins. Mutations of the S. meliloti nlpD gene caused decreased survival of cells in the stationary phase.

Original languageEnglish
Pages (from-to)292-295
Number of pages4
JournalArchives of Microbiology
Volume174
Issue number4
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • Alfalfa
  • Root colonization
  • Sinorhizobium meliloti
  • Stationary growth phase
  • Stress
  • Survival
  • bioS
  • nlpD

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