TY - JOUR
T1 - Molecular characterization of ochratoxin a producing strains of the genus Penicillium
AU - Castella, Gemma
AU - Ostenfeld Larsen, Thomas
AU - Cabanes, Javier
AU - Schmidt, Holger
AU - Alboresi, Alessandro
AU - Niessen, Ludwig
AU - Färber, Paul
AU - Geisen, Rolf
N1 - Funding Information:
This work was supported by the EU Project QLK1–CT-1999–01380 (European Commision, Quality of Life and Management of Living Resources Programme (QOL), Key Action 1 on Food, Nutrition and Health, Contract No. QLK1–CT-1999–01380).
PY - 2002
Y1 - 2002
N2 - Sixty-six strains classified as P. verrucosum based on morphological criteria were characterized by molecular methods like RAPD, AFLP and ITS sequencing. Two groups could be identified by RAPD and AFLP analyses. The two RAPD as well as the two AFLP groups were completely coincidental. Strains in the two groups differed in their ability to produce ochratoxin A, with group I containing mainly high producing strains, and group II containing moderate to non-producing strains. The strains from group I originate from foods, such as cheeses and meat products, while the strains from group II originate from plants. The ribosomal ITS1-5.8S-ITS2 sequences were similar, except for two single nucleotide exchanges in several strains of each group. A chemotaxonomical analysis of some of the strains identified differences between the groups in secondary metabolite production. Strains from group I possessed the chemotype of P. nordicum and strains from group II that of P. verrucosum. The differences at the RAPD and AFLP level, which parallel the chemotypic differences, are consistent with the recent reclassification of ochratoxin A producing penicillia to be either P. verrucosum or P. nordicum. The homolgy between the ITS sequences however indicates phylogenetic relationship between the two species.
AB - Sixty-six strains classified as P. verrucosum based on morphological criteria were characterized by molecular methods like RAPD, AFLP and ITS sequencing. Two groups could be identified by RAPD and AFLP analyses. The two RAPD as well as the two AFLP groups were completely coincidental. Strains in the two groups differed in their ability to produce ochratoxin A, with group I containing mainly high producing strains, and group II containing moderate to non-producing strains. The strains from group I originate from foods, such as cheeses and meat products, while the strains from group II originate from plants. The ribosomal ITS1-5.8S-ITS2 sequences were similar, except for two single nucleotide exchanges in several strains of each group. A chemotaxonomical analysis of some of the strains identified differences between the groups in secondary metabolite production. Strains from group I possessed the chemotype of P. nordicum and strains from group II that of P. verrucosum. The differences at the RAPD and AFLP level, which parallel the chemotypic differences, are consistent with the recent reclassification of ochratoxin A producing penicillia to be either P. verrucosum or P. nordicum. The homolgy between the ITS sequences however indicates phylogenetic relationship between the two species.
KW - AFLP typing
KW - Chemotyping
KW - ITS sequences
KW - Ochratoxin
KW - Penicillium nordicum
KW - Penicillium verrucosum
KW - RAPD typing
UR - http://www.scopus.com/inward/record.url?scp=0035989117&partnerID=8YFLogxK
U2 - 10.1078/0723-2020-00094
DO - 10.1078/0723-2020-00094
M3 - Article
C2 - 12086192
AN - SCOPUS:0035989117
SN - 0723-2020
VL - 25
SP - 74
EP - 83
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 1
ER -