Molecular and functional characterisation of the zebrafish (Danio rerio) PEPT1-type peptide transporter

Tiziano Verri; Gabor Kottra; Alessandro Romano; Natascia Tiso; Mark Peric; Michele Maffia; Michael Boll; Francesco Argenton; Hannelore Daniel; Carlo Storelli

doi:10.1016/S0014-5793(03)00759-2

Molecular and functional characterisation of the zebrafish (Danio rerio) PEPT1-type peptide transporter

Tiziano Verri, Gabor Kottra, Alessandro Romano, Natascia Tiso, Mark Peric, Michele Maffia, Michael Boll, Francesco Argenton, Hannelore Daniel, Carlo Storelli

Life Sciences

Research output: Contribution to journal › Article › peer-review

136 Scopus citations

Abstract

We report the molecular and functional characterisation of a novel peptide transporter from zebrafish, orthologue to mammalian and avian PEPT1. Zebrafish PEPT1 is a low-affinity/high-capacity system. However, in contrast to higher vertebrate counterparts in which maximal transport activity is independent of extracellular pH, zebrafish PEPT1 maximal transport rates unexpectedly increase at alkaline extracellular pH. Zebrafish pept1 is highly expressed in the proximal intestine since day 4 post-fertilisation, thus preceding functional maturation of the gut, first feeding and complete yolk resorption. Zebrafish PEPT1 might help to understand the evolutionary and functional relationships among vertebrate peptide transporters. Moreover, zebrafish pept1 can be a useful marker for screening mutations that affect gut regionalisation, differentiation and morphogenesis.

Original language	English
Pages (from-to)	115-122
Number of pages	8
Journal	FEBS Letters
Volume	549
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(03)00759-2
State	Published - 14 Aug 2003

Keywords

Gut morphogenesis
Intestine
Peptide transport
Two-electrode voltage clamp
Whole-mount in situ hybridisation
Xenopus laevis oocytes

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10.1016/S0014-5793(03)00759-2

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title = "Molecular and functional characterisation of the zebrafish (Danio rerio) PEPT1-type peptide transporter",

abstract = "We report the molecular and functional characterisation of a novel peptide transporter from zebrafish, orthologue to mammalian and avian PEPT1. Zebrafish PEPT1 is a low-affinity/high-capacity system. However, in contrast to higher vertebrate counterparts in which maximal transport activity is independent of extracellular pH, zebrafish PEPT1 maximal transport rates unexpectedly increase at alkaline extracellular pH. Zebrafish pept1 is highly expressed in the proximal intestine since day 4 post-fertilisation, thus preceding functional maturation of the gut, first feeding and complete yolk resorption. Zebrafish PEPT1 might help to understand the evolutionary and functional relationships among vertebrate peptide transporters. Moreover, zebrafish pept1 can be a useful marker for screening mutations that affect gut regionalisation, differentiation and morphogenesis.",

keywords = "Gut morphogenesis, Intestine, Peptide transport, Two-electrode voltage clamp, Whole-mount in situ hybridisation, Xenopus laevis oocytes",

author = "Tiziano Verri and Gabor Kottra and Alessandro Romano and Natascia Tiso and Mark Peric and Michele Maffia and Michael Boll and Francesco Argenton and Hannelore Daniel and Carlo Storelli",

note = "Funding Information: This investigation was supported by the Italian Ministry for the Agriculture and Forestry Policies (Fifth Plan on Fisheries and Aquaculture) and National Research Council (CNR Target Project on Biotechnology). ",

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doi = "10.1016/S0014-5793(03)00759-2",

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T1 - Molecular and functional characterisation of the zebrafish (Danio rerio) PEPT1-type peptide transporter

AU - Verri, Tiziano

AU - Kottra, Gabor

AU - Romano, Alessandro

AU - Tiso, Natascia

AU - Peric, Mark

AU - Maffia, Michele

AU - Boll, Michael

AU - Argenton, Francesco

AU - Daniel, Hannelore

AU - Storelli, Carlo

N1 - Funding Information: This investigation was supported by the Italian Ministry for the Agriculture and Forestry Policies (Fifth Plan on Fisheries and Aquaculture) and National Research Council (CNR Target Project on Biotechnology).

PY - 2003/8/14

Y1 - 2003/8/14

N2 - We report the molecular and functional characterisation of a novel peptide transporter from zebrafish, orthologue to mammalian and avian PEPT1. Zebrafish PEPT1 is a low-affinity/high-capacity system. However, in contrast to higher vertebrate counterparts in which maximal transport activity is independent of extracellular pH, zebrafish PEPT1 maximal transport rates unexpectedly increase at alkaline extracellular pH. Zebrafish pept1 is highly expressed in the proximal intestine since day 4 post-fertilisation, thus preceding functional maturation of the gut, first feeding and complete yolk resorption. Zebrafish PEPT1 might help to understand the evolutionary and functional relationships among vertebrate peptide transporters. Moreover, zebrafish pept1 can be a useful marker for screening mutations that affect gut regionalisation, differentiation and morphogenesis.

AB - We report the molecular and functional characterisation of a novel peptide transporter from zebrafish, orthologue to mammalian and avian PEPT1. Zebrafish PEPT1 is a low-affinity/high-capacity system. However, in contrast to higher vertebrate counterparts in which maximal transport activity is independent of extracellular pH, zebrafish PEPT1 maximal transport rates unexpectedly increase at alkaline extracellular pH. Zebrafish pept1 is highly expressed in the proximal intestine since day 4 post-fertilisation, thus preceding functional maturation of the gut, first feeding and complete yolk resorption. Zebrafish PEPT1 might help to understand the evolutionary and functional relationships among vertebrate peptide transporters. Moreover, zebrafish pept1 can be a useful marker for screening mutations that affect gut regionalisation, differentiation and morphogenesis.

KW - Gut morphogenesis

KW - Intestine

KW - Peptide transport

KW - Two-electrode voltage clamp

KW - Whole-mount in situ hybridisation

KW - Xenopus laevis oocytes

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DO - 10.1016/S0014-5793(03)00759-2

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AN - SCOPUS:0041562743

SN - 0014-5793

VL - 549

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EP - 122

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

Molecular and functional characterisation of the zebrafish (Danio rerio) PEPT1-type peptide transporter

Abstract

Keywords

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