Modulation of ABA responses by the protein kinase WNK8

Rainer Waadt; Esther Jawurek; Kenji Hashimoto; Yan Li; Martin Scholz; Melanie Krebs; Gereon Czap; Anne Hong-Hermesdorf; Michael Hippler; Erwin Grill; Jörg Kudla; Karin Schumacher

doi:10.1002/1873-3468.13315

Modulation of ABA responses by the protein kinase WNK8

Rainer Waadt, Esther Jawurek, Kenji Hashimoto, Yan Li, Martin Scholz, Melanie Krebs, Gereon Czap, Anne Hong-Hermesdorf, Michael Hippler, Erwin Grill, Jörg Kudla, Karin Schumacher

Chair of Botany

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Abscisic acid (ABA) regulates growth and developmental processes in response to limiting water conditions. ABA functions through a core signaling pathway consisting of PYR1/PYL/RCAR ABA receptors, type 2C protein phosphatases (PP2Cs), and SnRK2-type protein kinases. Other signaling modules might converge with ABA signals through the modulation of core ABA signaling components. We have investigated the role of the protein kinase WNK8 in ABA signaling. WNK8 interacted with PP2CA and PYR1, phosphorylated PYR1 in vitro, and was dephosphorylated by PP2CA. A hypermorphic wnk8-ct Arabidopsis mutant allele suppressed ABA and glucose hypersensitivities of pp2ca-1 mutants during young seedling development, and WNK8 expression in protoplasts suppressed ABA-induced reporter gene expression. We conclude that WNK8 functions as a negative modulator of ABA signaling.

Original language	English
Pages (from-to)	339-351
Number of pages	13
Journal	FEBS Letters
Volume	593
Issue number	3
DOIs	https://doi.org/10.1002/1873-3468.13315
State	Published - Feb 2019

Keywords

Arabidopsis thaliana
abscisic acid
protein kinase WNK8
protein phosphatase PP2CA
seed germination

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title = "Modulation of ABA responses by the protein kinase WNK8",

abstract = "Abscisic acid (ABA) regulates growth and developmental processes in response to limiting water conditions. ABA functions through a core signaling pathway consisting of PYR1/PYL/RCAR ABA receptors, type 2C protein phosphatases (PP2Cs), and SnRK2-type protein kinases. Other signaling modules might converge with ABA signals through the modulation of core ABA signaling components. We have investigated the role of the protein kinase WNK8 in ABA signaling. WNK8 interacted with PP2CA and PYR1, phosphorylated PYR1 in vitro, and was dephosphorylated by PP2CA. A hypermorphic wnk8-ct Arabidopsis mutant allele suppressed ABA and glucose hypersensitivities of pp2ca-1 mutants during young seedling development, and WNK8 expression in protoplasts suppressed ABA-induced reporter gene expression. We conclude that WNK8 functions as a negative modulator of ABA signaling.",

keywords = "Arabidopsis thaliana, abscisic acid, protein kinase WNK8, protein phosphatase PP2CA, seed germination",

author = "Rainer Waadt and Esther Jawurek and Kenji Hashimoto and Yan Li and Martin Scholz and Melanie Krebs and Gereon Czap and Anne Hong-Hermesdorf and Michael Hippler and Erwin Grill and J{\"o}rg Kudla and Karin Schumacher",

note = "Publisher Copyright: {\textcopyright} 2018 Federation of European Biochemical Societies",

year = "2019",

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doi = "10.1002/1873-3468.13315",

language = "English",

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AU - Waadt, Rainer

AU - Jawurek, Esther

AU - Hashimoto, Kenji

AU - Li, Yan

AU - Scholz, Martin

AU - Krebs, Melanie

AU - Czap, Gereon

AU - Hong-Hermesdorf, Anne

AU - Hippler, Michael

AU - Grill, Erwin

AU - Kudla, Jörg

AU - Schumacher, Karin

PY - 2019/2

Y1 - 2019/2

N2 - Abscisic acid (ABA) regulates growth and developmental processes in response to limiting water conditions. ABA functions through a core signaling pathway consisting of PYR1/PYL/RCAR ABA receptors, type 2C protein phosphatases (PP2Cs), and SnRK2-type protein kinases. Other signaling modules might converge with ABA signals through the modulation of core ABA signaling components. We have investigated the role of the protein kinase WNK8 in ABA signaling. WNK8 interacted with PP2CA and PYR1, phosphorylated PYR1 in vitro, and was dephosphorylated by PP2CA. A hypermorphic wnk8-ct Arabidopsis mutant allele suppressed ABA and glucose hypersensitivities of pp2ca-1 mutants during young seedling development, and WNK8 expression in protoplasts suppressed ABA-induced reporter gene expression. We conclude that WNK8 functions as a negative modulator of ABA signaling.

AB - Abscisic acid (ABA) regulates growth and developmental processes in response to limiting water conditions. ABA functions through a core signaling pathway consisting of PYR1/PYL/RCAR ABA receptors, type 2C protein phosphatases (PP2Cs), and SnRK2-type protein kinases. Other signaling modules might converge with ABA signals through the modulation of core ABA signaling components. We have investigated the role of the protein kinase WNK8 in ABA signaling. WNK8 interacted with PP2CA and PYR1, phosphorylated PYR1 in vitro, and was dephosphorylated by PP2CA. A hypermorphic wnk8-ct Arabidopsis mutant allele suppressed ABA and glucose hypersensitivities of pp2ca-1 mutants during young seedling development, and WNK8 expression in protoplasts suppressed ABA-induced reporter gene expression. We conclude that WNK8 functions as a negative modulator of ABA signaling.

KW - Arabidopsis thaliana

KW - abscisic acid

KW - protein kinase WNK8

KW - protein phosphatase PP2CA

KW - seed germination

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AN - SCOPUS:85059194271

SN - 0014-5793

VL - 593

SP - 339

EP - 351

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Modulation of ABA responses by the protein kinase WNK8

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