@inbook{22dd405b36844a998d1ac705aeb59f3f,
title = "Modifying Bacterial Artificial Chromosomes for Extended Genome Modification",
abstract = "Bacterial artificial chromosomes have been used extensively for the exploration of mammalian genomes. Although novel approaches made their initial function expendable, the available BAC libraries are a precious source for life science. Their comprising of extended genomic regions provides an ideal basis for creating a large targeting vector. Here, we describe the identification of suitable BACs from their libraries and their verification prior to manipulation. Further, protocols for modifying BAC, confirming the desired modification and the preparation of transfection into mammalian cells are given.",
keywords = "BAC Sanger sequencing, BAC fingerprinting, BAC libraries, Bacterial recombination, Genome modification, Instructions: Bacterial artificial chromosomes (BAC)",
author = "Hannah Auch and Nikolai Klymiuk and Petra Runa-Vochozkova",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2301-5_4",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "67--90",
booktitle = "Methods in Molecular Biology",
}