Mobility of the maize transposable element En/Spm in Arabidopsis thaliana

Guillermo H. Cardon, Monika Frey, Heinz Saedler, Alfons Gierl

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The autonomous element En-1 of the maize En/Spm transposable element system is capable of frequent somatic and germinal excision in the heterologous host Arabidopsis thaliana. The pattern of En-homologous transcripts generated in transgenic Arabidopsis resembles En transcription in maize. An excision reporter construct based on NPT-II gene (pKEn2) can be used reliably for the isolation of En-1 germinal revertants by seed germination on kanamycin-containing medium. Re-insertion after germinal excision is apparently frequent. A dSpm receptor element can be efficiently trans-activated in Arabidopsis either by En-1 or by expressing cDNAs of tnpA and tnpD. Excision and re-insertion of En/Spm take place with similar characteristics as in maize. This is the first description of En/Spm transposition in Arabidopsis and the parameters analysed here suggest that transposon tagging with En should be feasible in this species.

Original languageEnglish
Pages (from-to)773-784
Number of pages12
JournalPlant Journal
Volume3
Issue number6
DOIs
StatePublished - 1993
Externally publishedYes

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