MIR sequences recruit zinc finger protein ZNF768 to expressed genes

Michaela Rohrmoser, Michael Kluge, Yousra Yahia, Anita Gruber-Eber, Muhammad Ahmad Maqbool, Ignasi Forné, Stefan Krebs, Helmut Blum, Ann Katrin Greifenberg, Matthias Geyer, Nicolas Descostes, Axel Imhof, Jean Christophe Andrau, Caroline C. Friedel, Dirk Eick

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Mammalian-wide interspersed repeats (MIRs) are retrotransposed elements of mammalian genomes. Here,we report the specific binding of zinc finger protein ZNF768 to the sequence motif GCTGTGTG (N20) CCTCTCTG in the core region of MIRs. ZNF768 binding is preferentially associated with euchromatin and promoter regions of genes. Binding was observed for genes expressed in a cell type-specific manner in human B cell line Raji and osteosarcoma U2OS cells. Mass spectrometric analysis revealed binding of ZNF768 to Elongator components Elp1, Elp2 and Elp3 and other nuclear factors. The N-terminus of ZNF768 contains a heptad repeat array structurally related to the C-terminal domain (CTD) of RNA polymerase II. This array evolved in placental animals but not marsupials and monotreme species, displays species-specific length variations, and possibly fulfills CTD related functions in gene regulation. We propose that the evolution of MIRs and ZNF768 has extended the repertoire of gene regulatory mechanisms in mammals and that ZNF768 binding is associated with cell type-specific gene expression.

Original languageEnglish
Pages (from-to)700-715
Number of pages16
JournalNucleic Acids Research
Issue number2
StatePublished - 25 Jan 2019
Externally publishedYes


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