TY - JOUR
T1 - miR-191 modulates B-cell development and targets transcription factors E2A, Foxp1, and Egr1
AU - Blume, Jonas
AU - Ziętara, Natalia
AU - Witzlau, Katrin
AU - Liu, Yanshan
AU - Sanchez, Oskar Ortiz
AU - Puchałka, Jacek
AU - Winter, Samantha J.
AU - Kunze-Schumacher, Heike
AU - Saran, Namita
AU - Düber, Sandra
AU - Roy, Bishnudeo
AU - Weiss, Siegfried
AU - Klein, Christoph
AU - Wurst, Wolfgang
AU - Łyszkiewicz, Marcin
AU - Krueger, Andreas
N1 - Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2019/1
Y1 - 2019/1
N2 - The interdependence of posttranscriptional gene regulation via miRNA and transcriptional regulatory networks in lymphocyte development is poorly understood. Here, we identified miR-191 as direct upstream modulator of a transcriptional module comprising the transcription factors Foxp1, E2A, and Egr1. Deletion as well as ectopic expression of miR-191 resulted in developmental arrest in B lineage cells, indicating that fine tuning of the combined expression levels of Foxp1, E2A, and Egr1, which in turn control somatic recombination and cytokine-driven expansion, constitutes a prerequisite for efficient B-cell development. In conclusion, we propose that miR-191 acts as a rheostat in B-cell development by fine tuning a key transcriptional program.
AB - The interdependence of posttranscriptional gene regulation via miRNA and transcriptional regulatory networks in lymphocyte development is poorly understood. Here, we identified miR-191 as direct upstream modulator of a transcriptional module comprising the transcription factors Foxp1, E2A, and Egr1. Deletion as well as ectopic expression of miR-191 resulted in developmental arrest in B lineage cells, indicating that fine tuning of the combined expression levels of Foxp1, E2A, and Egr1, which in turn control somatic recombination and cytokine-driven expansion, constitutes a prerequisite for efficient B-cell development. In conclusion, we propose that miR-191 acts as a rheostat in B-cell development by fine tuning a key transcriptional program.
KW - B cells
KW - Lymphocyte development
KW - Transcriptional factors
KW - miR-191
KW - miRNA
UR - http://www.scopus.com/inward/record.url?scp=85055250220&partnerID=8YFLogxK
U2 - 10.1002/eji.201847660
DO - 10.1002/eji.201847660
M3 - Article
C2 - 30281154
AN - SCOPUS:85055250220
SN - 0014-2980
VL - 49
SP - 121
EP - 132
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 1
ER -